藏雪莲多糖通过P38丝裂原激活蛋白激酶通路减轻中波紫外线辐射后皮肤角质形成细胞凋亡引起炎性损伤的研究  

Alleviation of Inflammatory Damage Induced by the Apoptosis of Ha Ca T Cells After Ultraviolet B Radiation by Saussurea Tridactyla Sch.- Bip. Polysaccharides Through P38MAPK Passage

作　　者：郭砚[1] 孙娟[2] 王丽雯[2] 

机构地区：[1]青海大学附属医院皮肤病与性病科,青海省西宁市810001 [2]青海大学研究生院

出　　处：《中国全科医学》2015年第27期3314-3319,共6页Chinese General Practice

基　　金：青海省(应用)基础研究计划项目(2013-Z-730)

摘　　要：目的探讨藏雪莲(SSB)多糖是否通过P38丝裂原激活蛋白激酶(P38MAPK)通路减轻中波紫外线(UVB)辐射后皮肤角质形成细胞(Ha Ca T细胞)凋亡引起炎性损伤。方法 2014年10月—2015年3月,提取SSB多糖,体外培养Ha Ca T细胞,将Ha Ca T细胞分为低剂量辐射组(30 m J/cm2,辐射1 h,A组)和高剂量辐射组(60 m J/cm2,辐射1 h,B组);将A组和B组又分别分为对照组(1组)、辐射组(UVB,2组)、低剂量SSB多糖组(UVB+SSB 10 mg/ml,3组)和高剂量SSB多糖组(UVB+SSB 40 mg/ml,4组)。采用酶联免疫吸附实验(ELISA)法检测并比较白介素6(IL-6)、肿瘤坏死因子α(TNF-α)、P38MAPK、P53、B细胞淋巴瘤因子2(Bcl-2)、Bcl-2相关X(Bax)、半胱氨酸蛋白酶3(Caspase-3)水平。结果 A2组IL-6、TNF-α、P38MAPK、P53、Bax、Caspase-3水平高于A1组,Bcl-2水平低于A1组(P<0.05);A3组IL-6、TNF-α、P38MAPK、Caspase-3水平高于A1组(P<0.05);A4组TNF-α、Bcl-2水平高于A1组(P<0.05);A3组、A4组IL-6、TNF-α、P38MAPK、P53、Bax、Caspase-3水平低于A2组,Bcl-2水平高于A2组(P<0.05);A4组IL-6、TNF-α、Caspase-3水平低于A3组,Bcl-2水平高于A3组(P<0.05)。B2组IL-6、TNF-α、P38MAPK、P53、Bax、Caspase-3水平高于B1组,Bcl-2水平低于B1组(P<0.05);B3组IL-6、TNF-α水平高于B1组,Bcl-2水平低于B1组(P<0.05);B4组IL-6、Bcl-2水平高于B1组,TNF-α、Caspase-3水平低于B1组(P<0.05);B3组、B4组IL-6、TNF-α、P38MAPK、P53、Bax、Caspase-3水平低于B2组,Bcl-2水平高于B2组(P<0.05);B4组IL-6、TNF-α、Caspase-3水平低于B3组,Bcl-2水平高于B3组(P<0.05)。结论 SSB多糖通过P38MAPK通路减少UVB辐射后Ha Ca T细胞凋亡,进而减轻Ha Ca T细胞的炎性损伤。Objective To investigate whether Saussurea Tridactyla Sch. - Bip. （ SSB ） polysaccharides could alleviate inflammatory damage induced by the apoptosis of HaCaT cells after ultraviolet B（ UVB） radiation through P38MAPK passage. Methods From October 2014 to March 2015,SSB polysaccharideswere extracted,and HaCaT cells were cultured in vitro and were respectively divided into low dose radiation group（30 mJ/cm2 ,radiation 1 h,group A）,high dose radiation group （60 mJ/cm2,radiation 1 h,group B）. Each of the two groups were further divided into control group（group 1）,UVB radiation group（UVB,group 2）,low dose SSB polysaccharide group（UVB＋SSB 10 mg/ml,group 3）,high dose SSB polysaccharide group（UVB＋SSB 40 mg/ml,group 4）. ELISA method was used to test and compare the levels of IL-6,TNF-α,P38MAPK, P53,Bcl-2,Bax and Caspase-3. Results Group A2 was higher（P〈0. 05）in the levels of IL-6,TNF-α,P38MAPK,nbsp;P53,Bax and Caspase-3 and lower（P〈0. 05）in the level of Bcl-2 than group A1;group A3 was higher（P〈0. 05）than group A1 in the levels of IL-6,TNF-α,P38MAPK and Caspase-3;group A4 was higher（P〈0. 05）than group A1 in the levels of TNF-α and Bcl-2;group A3 and group A4 were lower（P〈0. 05）in the levels of IL-6,TNF-α,P38MAPK, P53,Bax and Caspase-3 and higher（P〈0. 05）in the level of Bcl-2 than group A2;group A4 was lower（P〈0. 05）in the levels of IL-6,TNF-αand Caspase-3 and higher（P〈0. 05）in the level of Bcl-2 than group A3. Group B2 was higher（P〈0. 05）in the levels of IL-6,TNF-α,P38MAPK,P53,Bax and Caspase-3 and lower（P〈0. 05）in the level of Bcl-2 than group B1;group B3 was higher（P〈0. 05）in the levels of IL-6 and TNF-αand lower（P〈0. 05）in the level of Bcl-2 than group B1;group B4 was higher（P〈0. 05）in the levels of IL-6 and Bcl-2 and lower（P〈0. 05）in the level of TNF-αand Caspase-3 than group B1;group B3 and group B4 were lower（P〈0. 05）in the levels of IL-6,TNF-α,P38MAPK, P53,Bax and Caspase-3 and hi

关 键 词：紫外线 藏雪莲多糖 皮肤角质形成细胞 白细胞介素6 肿瘤坏死因子α 丝裂原激活蛋白激酶类 淋巴瘤 B细胞 BCL-2相关X蛋白质 半胱氨酸蛋白酶类 

分 类 号：R122.25[医药卫生—环境卫生学]