Sunfan叶绿素及荧光参数对矮壮素的响应  

Response of Chlorophyll and Chlorophyll Fluorescence Parameters of Scaevola aemula 'Sunfan' to Chlorocholine Chloride

作　　者：李源[1] 何丙辉[1] 黄小辉[2] 毛文韬[1] 于传 秦华军[1] 曾清苹 苏健波 

机构地区：[1]西南大学资源环境学院/三峡库区生态环境教育部重点实验室,重庆400715 [2]重庆市林业科学研究院/三峡库区森林生态保护与恢复重庆市市级重点实验室,重庆400036 [3]湖南省湘潭市林业局,湖南湘潭411100 [4]重庆市美樱时代生态农业开发有限责任公司,重庆400064

出　　处：《西南大学学报（自然科学版）》2015年第8期20-27,共8页Journal of Southwest University(Natural Science Edition)

基　　金：国家林业局公益性行业科研专项(201104043);重庆市科技攻关重点项目(CSTC2009AB1115);西南大学青年基金项目(SWU208048);西南大学生态学重点学科"211工程"三期建设项目

摘　　要：以1年生澳大利亚‘太阳扇’(Scaevola aemula‘Sunfan’)扦插苗为试验材料,研究了(10,50,100,200,300)mg/L矮壮素(CCC)处理对Sunfan叶片叶绿素a(Cha),叶绿素b(Chb),叶绿素总量(Chl)和叶绿素荧光参数[PSⅡ的最大光化学效率Fv/Fm,PSⅡ天线转化效率Fv′/Fm′,潜在光化学活性Fv/Fo,实际光化学效率ΦPSⅡ,电子传递速率ETR,光化学猝灭qP,非光化学猝灭NPQ,PSⅡ处调节性能量耗散的量子产量Y(NPQ)]的影响.结果表明:(10,50,100,200,300)mg/L CCC处理均可显著提高Sunfan叶片的Cha,Chb,Chl,Fv/Fm,Fv′/Fm′,Fv/Fo,ΦPSⅡ,ETR,qP(p<0.05),且均随着CCC质量浓度的增加呈先增大后减小趋势;CCC处理可显著降低Y(NPQ),NPQ(p<0.05),且均随着CCC质量浓度的增加呈递减趋势.当质量浓度为200 mg/L时,Cha,Chb,Chl,Fv/Fm,Fv′/Fm′,Fv/Fo,ΦPSⅡ,ETR达到最大值.相关分析表明,Cha,Chb,Chl均与Fv/Fm,Fv′/Fm′,Fv/Fo,ETR,ΦPSⅡ,qP呈极显著正相关(p<0.01),但与NPQ均呈负相关,与Y(NPQ)均呈显著负相关(p<0.05).由回归分析可知,Cha随着Fv/Fm值,qP值的增加而增加;Chb随着Fv/Fm值的增加而增加;Chl随着Fv/Fm值,qP值的增加而增加.One-year-old Scaevola aemula ＇Sunfan＇ cutting seedlings were foliar sprayed with CCC at 0 （control）, 10, 50, 100, 200 and 300 mg/L to investigate the effects of CCC on chlorophyll a （Ca）, chlorophyll b （Cb）, total chlorophyll （CT）, maximum photochemical efficiency of PS Ⅱ （Fv/Fm）, effective photochemical quantum yield of PS Ⅱ （Fv＇/Fm＇）, potential activities of PS Ⅱ （Fv/Fo）, effective photochemical quantum yield of PSH （ФPS Ⅱ ）, electron transport rate （ETR）, photochemical quench （qP）, non-photochemical quench （NPQ） and quantum yield of non-photochemical quench （Y（NPQ）） in the leaves. The results showed that all the CCC treatments significantly enhanced Ca, Cb, CT, Fv/Fm, Fv＇/ Fm＇, Fv/Fo, qSPS Ⅱ , ETR and qP（p〈0. 05）of Sunfan leaves and showed an increase first with increas- ing CCC concentration, followed by a decrease afterwards, and steadily decreased Y（NPQ） and NPQ （p 〈 0.05）of Sunfan leaves with increasing CCC concentration. Ca, Cb, CT, Fv/Fm, Fv＇/Fm＇, Fv/Fo, ФPS Ⅱ and ETR reached their maximum with 200 mg/L CCC. Correlation analysis indicated that Ca, Cb and CT were in highly significant positive correlations （p〈0.01） with Fv/Fm, Fv＇/Fm＇, Fv/Fo, ETR, ФPS Ⅱ and qP and were in negative correlations with NPQ, and in significant negative correlation with Y（NPQ） （p〈0.05）. According to regression analysis, Ca tended to increase with increasing Fv/Fm and qP, Cb tended to increase with increasing Fv/Fm and qP and CT tended to increase with increasing Fv/Fm and qP.

关 键 词：澳大利亚'太阳扇’ 矮壮素 叶绿素 叶绿素荧光参数 

分 类 号：S718.45[农业科学—林学]