球孢白僵菌Bb1287菌株对扶桑绵粉蚧毒力的测定  

Determination of Virulence of Beauveria bassiana Bb1287 to Phenacoccus solenopsis Tinsley

作　　者：袁盛勇[1,2] 孔琼[1,2] 薛春丽[1,2] 沈登荣[1,2] 张宏瑞[3] 陈斌[3] 何超[1,2] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661199 [2]云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661199 [3]云南农业大学植物保护学院,昆明650201

出　　处：《西南大学学报（自然科学版）》2015年第8期47-52,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(30860005);公益性行业(农业)科研专项(201103026);红河学院植物保护硕士授权点建设项目

摘　　要：为明确球孢白僵菌(Beauveria bassiana)Bb1287菌株对扶桑绵粉蚧的侵染能力.采用点滴法测定了球孢白僵菌Bb1287菌株对扶桑绵粉蚧1龄若虫,2龄若虫,3龄若虫和成虫的毒力.结果表明:在2.0×105,2.0×106,2.0×107和2.0×108个/mL 4个浓度的分生孢子液对扶桑绵粉蚧(Phenacoccus solenopsis Tinsley)的若虫和成虫具有很高的毒力.在分生孢子液2.0×108个/mL浓度时1龄若虫,2龄若虫,3龄若虫和成虫的累计校正死亡率分别是(88.52±13.16)%,(83.91±14.04)%,(79.25±9.39)%和(77.39±7.20)%.扶桑绵粉蚧1龄若虫,2龄若虫,3龄若虫和雌成虫的致死中浓度(LC50)分别为(3.2367±0.06)×105,(2.6070±0.06)×106,(3.3943±0.06)×106和(3.4239±0.06)×106个/mL,致死中时间(LT50)分别是(4.09±1.42),(4.70±0.78),(5.28±0.36)和(5.19±0.94)d.得出球孢白僵菌Bb1287菌株对扶桑绵粉蚧的若虫和成虫具有较强的毒力,可作为扶桑绵粉蚧生物制剂开发应用菌株.Objective： To investigate the infection ability of Beauveria bassiana Bb1287 to Phenacoccus solenopsis Tinsley. Methods：Virulence of B. bassiana strain Bb1287 to 1st instar larvae, 2nd instar larvae, 3rd instar larvae and adults of Ph. solenopsis was measured using topical application. Results： B. bassiana Bb1287 applied at 2.0 × 10^5 , 2.0 × 10^6 , 2.0 × 10^7 and 2.0 × 10^8 spores/mL showed high virulence to the larvae and adults of Ph. solenopsis. The adjusted accumulative mortality rate of the 1st instar larvae, 2nd instar larvae, 3rd instar larvae and adults of Ph. solenopsis was （88. 52 ±13. 16） %, （83. 91 ± 14.04） %, （ 79.25 ± 9. 39 ） % and （77.39 ±7.20） %, respectively, in the treatment of 2.0 ± 10^8 spores/mL. The median lethal concentration （LC50） of the 1st, 2nd and 3rd instar larvae and adults of the pest was （3. 236 7±0.06） ×10^5, （2. 607 0±0.06） ×10^6, （3. 394 3±0.06） ×10^6 and （3. 423 9±0.06） × 10^6 spores/mL, respectively; and the median lethal time （LT50） was （4.09 ± 1.42）, （4.70 ± 0.78）, （5.28 ± 0.36） and （5. 19±0.94） d, respectively. Conclusions： The B. bassiana strain Bb1287 is virulent against the larvae and adults of Ph. solenopsis and can be a potential strain for the development of biological control agents against Ph. solenopsis.

关 键 词：球孢白僵菌 扶桑绵粉蚧 毒力 

分 类 号：Q965.9[生物学—昆虫学]