放射创伤强度与主要组织相容性复合体缺失相关的自身免疫移植物抗宿主病的相关性研究  

Lack of major histocompatibility complex induced autoimmune graft-versus-host disease is related to radiation dose

作　　者：李晴[1] 郭锦华[2] 李晓帆[3,4] 

机构地区：[1]福建省立医院妇产科,福州350001 [2]福建医科大学附属协和医院放疗科,福州350001 [3]福建医科大学附属协和医院血液科,福州350001 [4]福建省血液病研究所,福建省血液病学重点实验室,福州350001

出　　处：《创伤与急诊电子杂志》2015年第3期18-22,共5页Journal of Trauma and Emergency(Electronic Version)

基　　金：国家自然科学基金青年项目(81200400);教育部博士点基金(20123518120006);福建省高校杰出青年科研人才培育计划(JA14130)

摘　　要：目的研究放射创伤强度与主要组织相容性复合体缺失相关的自身免疫移植物抗宿主病的关系。方法受鼠予不同的全身放射强度进行全身放射,放射后回输T淋巴细胞去除的骨髓细胞。将受鼠分两组:1WT组:回输来自野生型C57BL/6(H2b)细胞106/只;2 MHC-/-组:回输来自主要组织相容性复合体(major histocompatibility complex,MHC)Ⅱ类分子缺失的C57BL/6(H2b)细胞106/只。移植后记录小鼠体重、观察移植小鼠GVHD典型症状及生存率等;GVHD发病小鼠行病理切片确诊。结果 1 1000rad的全身放射强度剂量可以在异基因MHC全不相合移植(H2b→H2d)动物模型中诱导出的MHCⅡ类分子缺失相关的自身免疫GVHD。2 1000rad的放射强度,MHC-/-组小鼠移植后体重下降明显、GVHD评分高、组织病理见大量的淋巴细胞浸润(P<0.05)。3减弱为800rad的放射强度两组小鼠生存率及体重无统计学差别,未见明显弓背、脱毛、腹泻、脱皮等GVHD症状(P>0.05)。Objective To explore the relationship between radiation dose and lack of major histocompatibility complex induced autoimmune graft-versus-host disease（gVHd）.Method mice were conditioned with different doses of total body irradiation（TBi） and transplanted with T-cell depleted（Tcd） bone marrow; they were assigned to two groups. mice in WT group were injected with 106 Tcd bone marrow cells while mice in mHc-/- group were injected with 106 Tcd bone marrow cells. loss of body weight, gVHd clinical scores and the survival rate of each group were monitored.Histology was used to analyze the severity of gVHd.Result①We found that radiation doses at 1000rad was able to induce autoimmune graft-versus-host disease caused by lack of major histocompatibility complex in a mHc mismatched allo-HcT mouse model.②under 1000rad radiation, mice in mHc-/- group showed higher clinical score and shorter survival time;histopathology showed vast lymphocytes infiltration （P〈0.05）.③under 800rad radiation, there was no significant difference between those two groups in body weight or overall survival rate;neither group showed gVHd（P＆gt;0.05）.Conclusion lack of major histocompatibility complex induced autoimmune graft-versus-host disease is related to radiation doses.

关 键 词：放射创伤强度 移植物抗宿主病 主要组织相容性复合体 自身免疫 动物模型 

分 类 号：R818.02[医药卫生—放射医学]