赤芍总苷诱导扁平苔藓中T细胞免疫和凋亡机制的研究  

Effect on the level of T cells immunity and mechanism of apoptosis in the Oral lichen planus patients induced by the total paeony glycoside

作　　者：杨霞[1] 黄吉燕[2] 王明[1] 向学熔[1] 李洁仪[1] 曾兴琪[1] 李丛华[1] 

机构地区：[1]重庆医科大学附属口腔医院/口腔疾病与生物医学重庆市重点实验室,400015 [2]上海中医药大学附属曙光医院口腔科,200021

出　　处：《重庆医学》2015年第26期3622-3624,3627,共4页Chongqing medicine

基　　金：重庆市卫生局中医药科研计划项目(2010-2-67);重庆市卫生局医学科研项目(2012-1-056)

摘　　要：目的观察扁平苔藓(OLP)患者赤芍总苷(TPG)治疗前后T细胞免疫表达水平的变化,探讨影响细胞凋亡的机制。方法应用TPG局部治疗20例OLP患者,观察其治疗后的临床效果;且在治疗前后,较系统地对TPG诱导细胞凋亡情况进行比较;探讨OLP中的细胞凋亡情况及CD4+、CD8+T细胞和CD4+/CD8+比例的变化,分析TPG诱导OLP患者中的T细胞免疫与细胞凋亡的关系。结果 TPG治疗扁平苔藓临床疗效明显,治疗后患者OLP病变组织处网纹面积减小,糜烂面大小减少,患者疼痛指数明显降低。治疗后CD4+和CD8+表达较治疗前显著下降。结论 TPG的治疗可能是通过固有层中诱导炎性T细胞的凋亡,T细胞免疫受到调节,从而减轻OLP炎症的发生和发展,起到治疗OLP的作用。Objective Observe the effect of Total paeony glycoside（TPG）has on the T-cell immune action and apoptosis process of Oral lichen planus patients and analyze the mechanism. Methods 20 OLP patients are recruited into this experiment, each is treated with TPG,and their clinical effect is taken notes of. Observe the apoptosis of OLP lesions from each of the patients before and after the treatment, compare the apoptosis condition and the expression of CD4＋ , CD8＋ , and the CD4＋/CD8＋ ratio, to analyze the role TPG plays on the immune expression of T-cell and the effect on apoptosis process. Results TPG improve the clini- cal manifestation of OLP patients significantly, resulting in the decrease of reticulum and erosive areas, and the pain index is de- creased obviously. The expression of CD4＋ and CD8＋ are decreased in OLP lesions after the treatment of TPG. Conclusion TPG may induce the apoptosis of the T-cell in lamina propria of the OLP lesions to regulate the T-cell immune action of OLP patients, and slow the development of inflammatory process of OLP,and in that way,TPG shows its potential in treating OLP,and this ex periment can provide the primary evidence.

关 键 词：赤芍总苷 扁平苔藓 细胞免疫 细胞凋亡 

分 类 号：R276.8[医药卫生—中医五官科学]