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出 处:《西南农业学报》2015年第4期1490-1496,共7页Southwest China Journal of Agricultural Sciences
基 金:国家自然科学基金项目(31160054);广西研究生教育创新计划项目(YCSZ2014031)
摘 要:采用水培法对木豆芽苗进行不同浓度铝(0~200μmol/L Al Cl3)处理0~72 h,通过测定根长,根尖胼胝质和铝含量对木豆的耐铝性进行了初期鉴定;苗期以1/5 Hoagland营养液水培并进行0~3 mmol/L的Al Cl3胁迫14 d,对根系结构、光合作用气体交换和叶绿素a荧光参数等进行研究。主要结果如下:芽期铝处理,桂木6号根尖积累胼胝质和铝含量低于桂木4号;苗期实验中,低浓度铝处理(≤1 mmol/L)促进木豆根系发育,根系活力升高,叶面积增加,净光合速率(Pn)增大,荧光参数Fm,Fm/Fo,Yield略有增加。高浓度铝处理(〉1 mmol/L)木豆根系生长受阻,根系活力、叶面积,Pn均较对照显著降低,Fo增大,Fm,Fm/Fo,Yield下降显著。桂木6号较桂木4号表现出更强的抗铝特性。In this study,the tolerances of two pigeon pea cultivas,Guimu 4( GM4) and Guimu 6( GM6) to Al were screened by measuring the root elongation,accumulations of callose and aluminum in root apex under stresses of different Al concentrations( 0- 200 μmol / L) and duration( 0- 72 h). And then,the two cultivars were cultured and stressed in 1 /5 Hoagland containing 0- 3 mmol / L Al Cl3 for 14 d. The morphological structure of pigeon pea root,photosynthetic gas exchange and chlorophyll a fluorescence characteristics were investigated. The results show that: Under low concentrations of aluminum( ≤1 mmol / L),the root growth was promoted with root activity increasing. Leaf area,the net photosynthetic rate( Pn) and chlorophyll a fluorescence parameters such as Fm,Fm/Fo,Yield have increased at different levels.In high level of Al stress. Root growth inhibition was significant. Leaf area,Pn,Fm,Fm/Fo,Yield have declined rapidly while Fo increased with increasing of aluminum concentration. In summary,GM6 has a stronger resistance to aluminum than GM4.
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