硝酸纤维素膜的再生处理及其吸附蛋白能力的研究  

Study on protein adsorption ability and the regeneration of nitrocellulose membrane

作　　者：李福刚 

机构地区：[1]上海奥普生物医药有限公司,上海201201

出　　处：《中国医学装备》2015年第9期14-17,共4页China Medical Equipment

基　　金：科技型中小企业技术创新基金(05C26213100490)"金标斑点法定量检测系统技术平台的产业化"

摘　　要：目的:解决硝酸纤维素膜老化在金标斑点定性或定量渗滤试验检测系统中带来的检测结果不准确的问题。方法:以两项胶体金斑点免疫渗滤试验——结核分枝杆菌抗体胶体金法定性检测(TB-DOT)和人绒毛膜促性腺激素金标法定量检测(HCG-DOT)为研究模式,对比3-甲基-1-丁醇处理前后的老化膜与正常膜的呈色结果、流速、吸附能力及稳定性等。结果:实验数据证实,处理后的硝酸纤维素膜流速提高14.4%,吸附能力明显增强,变异系数(CV)值均小于未处理的膜,稳定性良好,与新膜保持一致,表明再生处理的方法能够在一定程度上改善膜的性能。结论:本研究所使用的方法,不但解决了胶体金快速定量免疫化验测定试剂盒在生产中遇到的膜老化问题,也保证了产品在临床应用中具有更好的准确性。该解决方法具有一定普遍意义,同样适用于即时检验(POCT)类其他使用硝酸纤维素膜的诊断产品。Objective：To solve the inaccurate results caused by the aging of nitrocellulose membranes in qualitative or quantitative immuno-colloidal gold infiltration tests.Methods： Using qualitative TB-DOT and quantitative HCG-DOT immuno-colloidal gold filtration test systems, we compared the treated coloration, circulation velocity, adsorbing power and stability of nitrocellulose membrane of good quality, 3-methyl-1-butanol treated and untreated aged membranes. Results： Abundant results has approved that the regeneration of aged nitrocellulose membrane by 3-methyl-1-butanol treatment lead to 14.4% faster flow velocity, stronger absorption capability, better stability, and lower CV value, compared to untreated membranes.Conclusion：The mentioned method solves the problem caused by the aging of nitrocellulose membranes, and assures the accuracy of measurements. Furthermore, it is an universal solution that can be applied to other POCT products.

关 键 词：硝酸纤维素膜 再生处理 吸附能力 

分 类 号：R392.33[医药卫生—免疫学]