一贯煎汤剂对肝纤维化大鼠肝脏Ⅰ型胶原的影响  

Influence of Yiguanjian decoction on type I collagen in liver of rats with hepatic fibrosis

作　　者：白辰[1] 车念聪[1] 刘文兰[1] 赵晖[1] 司远[1] 

机构地区：[1]首都医科大学中医药学院,硕士研究生100069

出　　处：《环球中医药》2015年第8期901-904,共4页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81273668)

摘　　要：目的观察一贯煎汤剂对肝纤维化大鼠肝脏I型胶原的影响。方法将SD大鼠随机分为正常组、模型组、西药组、中药组。除正常组外,其余3组均建立CCl4四氯化碳诱导的肝纤维化模型。造模后,中药组以一贯煎汤剂灌胃、西药组以秋水仙碱溶液灌胃、正常组和模型组以生理盐水灌胃,灌胃次数为1次/天。灌胃4周后,通过蛋白电泳法检测I型胶原纤维蛋白的表达,通过免疫组化法检测大鼠肝组织细胞外I型胶原的沉积,分别以灰度值和积分光密度评价。结果与正常组相比,模型组肝组织细胞外I型胶原蛋白明显增加。与模型组相比,中药组及西药组肝组织细胞外I型胶原蛋白明显减少。结论一贯煎汤剂可减轻四氯化碳诱导的肝纤维化大鼠的肝组织I型胶原的表达及其在肝细胞外的沉积。Objective To investigate the influence of Yiguanjian decoction on type I collagen in liver of rats with hepatic fibrosis. Methods SD rats were randomly divided into control group, model group, western medicine group and Chinese medicine group. All the rats were used to establish hepatic fibrosis rat models in addition to rats in control group by using carbon tetrachloride （ CCl4 ） . After rat model establishment, rats in Chinese medicine group received gavage administration with Yiguanjian decoction, rats in western medicine group received gavage administration with colchicine solution, and rats in control and model groups received gavage administration with normal saline. After treatment for 4 weeks, protein e-lectrophoresis method was used to test the expression of type I collagen protein. Immunohistochemistry method was used to observe the extracellular deposition of type I collagen protein in hepatic tissue, and the depositionwas evaluated by gray value and integral optical density. Results Compared with control group, type I collagen protein of extracellular in hepatic tissue of rats in model group was increased significantly. Compared with model group, extracellular deposition of type I collagen protein in hepatic tissue of rats in Chinese medicine and western medicine groups was decreased significantly. Conclusion Yiguanjian decoction could relieve the expression of type I collagen protein and extracellular deposition of type I collagen protein in hepatic tissue of rats with hepatic fibrosis induced by CCl4 .

关 键 词：一贯煎 肝纤维化 I型胶原 

分 类 号：R657.31[医药卫生—外科学]