不同保存方法和研磨方式对玉米总DNA提取效果的影响  

Effects of Various Preservation and Grinding Methods on Extraction of Maize Total DNA

作　　者：宋红霞[1] 惠国强[2] 杨海鹏[2] 温琳[1] 孙毅[3,4] 刘小红[5] 张红梅[2,4] 

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西省农业科学院玉米研究所,山西忻州034000 [3]山西省农业科学院生物技术研究中心,山西太原030031 [4]农业部黄土高原作物基因资源和种质创制重点实验室,山西太原030031 [5]西华师范大学生命科学学院,四川南充637002

出　　处：《山西农业科学》2015年第9期1089-1092,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2013ZX08003-001)

摘　　要：以玉米自交系昌7-2为材料,采用改良CTAB法提取基因组DNA,分析不同保存和研磨方法等对样品提取效果的影响。通过A260/A280、琼脂糖凝胶电泳、PCR扩增,对提取的基因组DNA进行分析鉴定。结果表明,CTAB缓冲液保存叶片并在CTAB缓冲液中研磨叶片,得到的DNA效果最佳,同时这种方法也比较经济安全。Effects of various preservation and grinding methods, 18 treatment combinations, for extracting total DNA with modified CTAB method from the maize inbred line Chang 7-2 were studied. The quality and quantity of extracted DNA was examined using the A260/A280 value, the agarose gel electrophoresis, and PCR amplification. The results showed that CTAB buffer preservation and grinding leaves was the best way to extract DNA, and this treatment was relatively economical and safe.

关 键 词：玉米 叶片 基因组DNA 保存方法 研磨方法 

分 类 号：S513.01[农业科学—作物学]