一种罗丹明类Cu^(2+)荧光探针化合物的合成及性能研究  

Synthesis and properties study of a fluorescent probe compound based on rhodamine for Cu^(2+)

作　　者：翟荣佳 刘娅静[1,2] 赵鑫[1,2] 戚裕[1,2] 尹妮[1,2] 黄甜[1,2] 

机构地区：[1]苏州科技大学化学生物与材料工程学院,江苏苏州215009 [2]江苏省环境功能材料重点实验室,江苏苏州215009

出　　处：《化学研究与应用》2015年第9期1277-1282,共6页Chemical Research and Application

基　　金：江苏省研究生科研创新计划项目(CXLX12_0877)资助

摘　　要：以罗丹明B、水合肼和咔唑为原料,合成了一种新型的荧光增强型Cu2+荧光探针,即4-(N-咔唑基)苯亚甲基罗丹明B腙(CPMRH)。用FTIR、1H NMR和13C NMR对其分子结构进行了表征,并通过荧光光谱对探针的识别性能进行了研究。研究结果表明:探针CPMRH对水溶液中的Cu2+具有良好的选择识别性,且基本不受其他金属离子的影响;探针与Cu2+络合显示粉红色,可以作为一种裸眼检测的试剂用于溶液中Cu2+的检测;当λex=520 nm时,Cu2+水溶液与探针作用可显示橙红色荧光。且Cu2+浓度在1×10-5-5×10-5mol·L-1的范围内,探针的荧光强度与Cu2+浓度呈现出较好的线性关系;Cu2+的最低检出限为5.25×10-7mol·L-1;Job’s曲线表明,探针CPMRH与Cu2+的络合比为1∶1。A novel fluorescent probe compound,4-（ N-carbazolyl） phenyl methylene rhodamine B hydrazone（ CPMRH） for detecting Cu^2＋,was synthesized from rhodamine B,hydrazine hydrate and carbazole. The molecule structure of CPMRH was characterized by FTIR,^1 H NMR and ^13 C NMR,and the recognition properties of the CPMRH with metal ions had been investigated in aqueous solu-tion by the fluorescence spectrophotometry. The experimental results showed that CPMRH had high selectivity to Cu^2＋, and other metal ions had no effects on its recognition for Cu^2＋. After adding CPMRH to Cu^2＋ solution,the color of solution obviously change from colorless to pink,which demonstrated the probe could be used for“naked-eye”detection of Cu^2＋ in water. When the excitation wavelength was 520 nm,the solution of Cu^2＋ mixed with CPMRH showed the orange red fluorescence. When the concentration of Cu^2＋ was in the range of 1 × 10^-5-5 × 10^-5 mol·L^-1 ,there was a good linear relationship between the fluorescence intensity of CPM-RH and the concentration of Cu^2＋. The detection limit of CPMRH was 5. 25 × 10^-7 mol·L^-1 to Cu^2＋. The complexation ratio of CPM-RH/Cu^2＋ was 1∶1 from the method of Job’ s plot by fluorescence spectrophotometry.

关 键 词：荧光探针 罗丹明B CU^2+ 

分 类 号：O657[理学—分析化学]