氧化苦参碱体内外微透析回收率的研究  

Microdialysis recovery of oxymatrine in vitro and in vivo

作　　者：张岩[1,2] 何治芬 汤湛[1] 张雅雯[1] 王胜浩[1] 王俏[1] 

机构地区：[1]浙江省医学科学院药物研究所,杭州310013 [2]浙江工业大学药学院,杭州310014

出　　处：《中华中医药杂志》2015年第9期3108-3111,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省医学重点学科群项目(No.XKQ-010-001);浙江省科技计划项目(No.2012F10005);浙江省卫生高层次创新人才培养工程项目(No.XKQ010012008);省151人才工程第二层次项目~~

摘　　要：目的:研究氧化苦参碱浓度、灌流速度及试验方法对同心圆和线性两种微透析探针回收率的影响。方法:以氧化苦参碱为试验药物,分别测定药物不同浓度、不同灌流速度下,两种探针体外正向、反向回收率及体内反向回收率。结果:探针回收率均与药物浓度无关,与灌流速度成反比;两种探针各自的体外正向、反向回收率相似,且均大于体内反向回收率;同心圆探针的回收率大于线性探针。结论:探针回收率的测定是采用微透析研究药动学的重要试验,可为氧化苦参碱的体内药物浓度确定指明适用的方法。Objective： To evaluate the effects of drug concentration, flow rate and method on the recoveries of two different types of concentric cannula and linear microdialysis probes. Methods： Oxymatrine was selected as model drug. In vitro recovery of oxymatrine was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations. And in vivo recovery of oxymatrine was studied using retrodialysis methods. Results： The microdialysis recoveries of oxymatfine were inversely proportional to the perfusing rates, independent of oxymatrine concentrations. In vitro, the positive dialysis recoveries were similar to the retrodialysis recoveries. While the in vitro recoveries were greater than those in vivo. Under the same conditions, the recovery of concentric cannula probe was greater than that of linear probe. Conclusion： The determination of probe recovery is an important test for pharmacokinetics research on the basis of microdialysis, which indicates the appropriate and applicable way to measure the drug concentration of a certain kind of drug in the body.

关 键 词：微透析 氧化苦参碱 探针回收率 体内 体外 

分 类 号：R285[医药卫生—中药学]