清肠温中方对2,4,6-三硝基苯磺酸诱导结肠炎大鼠肠黏膜屏障的作用研究  

Effects of Qingchang Wenzhong Decoction on intestinal mucosa barrier in colitis rats induced by TNBS

作　　者：刘丽娟[1,2] 王允亮[1] 魏仕兵[1] 杨美娟[3] 李军祥[1] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]中日友好医院,北京100029 [3]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2015年第9期3266-3269,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京中医药大学创新团队资助项目(No.2011-CXTD-24);北京中医药大学大学生自主课题(No.2014-JYBZZ-XS-152)~~

摘　　要：目的:研究清肠温中方对2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎大鼠的疗效及作用机制。方法:以TNBS/乙醇灌肠诱导大鼠溃疡性结肠炎,造模成功后连续以清肠温中方(QCWZD)高、中、低剂量及柳氮磺吡啶(SASP)混悬液灌胃干预10d,取结肠,评价疾病活动指数(DAI),酶联免疫吸附法检测血清白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,免疫组织化学法检测occludin蛋白、带状闭合蛋白-1(ZO-1)在结肠黏膜的分布及表达。结果:模型组DAI评分、血清IL-6、TNF-α水平明显高于正常组(P<0.05),肠黏膜occludin、ZO-1表达总量低于正常组(P<0.01);清肠温中方各剂量组DAI水平较模型组明显下降(P<0.05);清肠温中方低剂量组血清IL-6水平均较模型组明显下降(P<0.05),清肠温中方高、中剂量组血清TNF-α水平均较模型组明显下降(P<0.05);清肠温中方高、中剂量组肠黏膜occludin、ZO-1蛋白表达总量显著高于模型组(P<0.01)。结论:清肠温中方能通过下调血清IL-6、TNF-α水平,升高肠黏膜occludin、ZO-1蛋白总量的表达,起到修复TNBS诱导的溃疡性结肠炎大鼠肠黏膜损伤的作用。Objective： To investigate the mechanism of Qingchang Wenzhong Decoction （QCWZD） in colitis rats induced by 2,4,6-trinitrobenzene sulfonic acid （TNBS）. Methods： Experimental colitis was induced in male sprague-dawley （SD） rats by intracolonic administration of TNBS/alcohol. High, medium, and low doses of QCWZD and salazosulfapyridine （SASP） was given by gavage route daily for 10 days. Disease activity index （DAI） was estimated. Serum IL-6 and TNF-α level was detected by Elisa assay. Occludin and ZO-1 in colonic mucosa were detected by immunological histological chemistry assay. Results： DAI and serum IL-6, TNF-α level were significantly higher in TNBS-treated rats than normal group （P〈0.05）, while expression of occluding and ZO-1 was significantly reduced （P〈0.01）. DAd in QCWZD groups （1 200mg/kg, 600mg/kg, 300mg/kg） decreased significantly compared with model group （P〈0.05）. Serum IL-6 expression was reduced in low dose QCWZD （300mg/Kg） group （P〈0.05）, and TNF-α expression was reduced in high and medium dose QCWZD （1200mg/kg, 600mg/kg） groups. Expression of occludin and ZO-1 in colonic mucosa was significantly increased in QCWZD （1200mg/kg, 600mg/kg） groups than model group （P〈0.01）. Conclusion： QCWZD could repair colonic mucosal damage of TNBS-induced colitis rats by down-regulating serum IL-6, TNF-α expression and increasing occludin and ZO-1 expression in colonic mucosa.

关 键 词：清肠温中方 溃疡性结肠炎 细胞因子 紧密连接 白介素-6 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]