miR-138对乳腺上皮细胞蛋白激酶C的调控及对β-酪蛋白分泌的影响  

Effect of miR-138 on protein kinase C and β-casein in mammary epithelial cells

作　　者：王春梅[1] 李晔[1] 佟慧丽[1] 李惠铭[1] 万中英[1] 李庆章[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2015年第9期8-10,共3页China Dairy Industry

基　　金：黑龙江省教育厅重点项目(1154z1004)

摘　　要：Mi RNA在细菌、真菌、哺乳动物等许多生物体的生命活动中发挥着极广泛的调控作用。应用乳腺上皮细胞为实验模型,采用脂质体转染法,添加mi R-138超表达子和mi R-138抑制子发现其调控蛋白激酶C的表达,通过构建mi R-138基因序列的双荧光素酶报告载体分析了mi R-138与靶基因蛋白激酶C之间的关系,采用Western印迹方法检测相关基因蛋白的表达变化.结果表明,mi R-138在乳腺细胞和组织中的表达与蛋白激酶C呈现负关联,mi R-138可通过调控蛋白激酶C酶的表达,从而调控乳腺功能的变化。MiRNA plays an extremely wide range of regulatory roIe in bacteria, fungi, and mammals. Application of mammary gland epitheli- al cells as experimental model, using liposome transfection method, add the miR-138 mimics and miR-138 inhibiter found its regulate the expression of protein kinase C, lnciferase report carrier was used to analyze the relationship with miR-138 and PKC; Western Blot was used to testing changes of protein kinase C. The results show that the miR - 138 in the tissue and mammary gland cells has a negative relation- ship expression of protein kinase C, miR-138 could be regulated by the expression of protein kinase C, thus regulate the function of mam- mary gland.

关 键 词：乳腺发育泌乳 miR-138 PKC 

分 类 号：Q936[生物学—微生物学]