参附注射液对脓毒症大鼠心肌细胞凋亡及Bcl-2表达的影响  

Effect of Shenfu Injection on the Cardiomyocyte Apoptosis and the Expression of Bcl-2 in Rat with Sepsis

作　　者：胡丹丹[1] 楼黎明[1] 徐慧连[1] 

机构地区：[1]浙江中医药大学附属第三医院,浙江杭州310005

出　　处：《中国中医急症》2015年第9期1540-1542,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江中医药大学科研项目(2010LX22);浙江中医药大学附属第三医院一般项目(ZS10CA15)

摘　　要：目的观察参附注射对脓毒症大鼠心肌细胞凋亡及Bcl-2蛋白表达的影响,探讨参附注射液抗脓毒症心肌损伤的相关机制。方法将24只雄性SD大鼠随机分为3组:假手术组、模型组和参附组,以盲肠结扎穿孔(CLP)法建立脓毒症大鼠动物模型,观察各组大鼠心肌酶谱变化;凋亡原位末端标记法(TUNEL)检测心肌细胞凋亡指数,以RT-PCR法检测心肌细胞Bcl-2 m RNA表达。结果参附组大鼠心肌酶谱升高程度较模型组低(P<0.05),模型组较假手术组心肌酶水平高(P<0.05);参附组大鼠心肌细胞TUNEL评分平均光密度(MOD)较模型组明显降低(P<0.05),模型组较假手术组升高(P<0.05);模型组心肌细胞Bcl-2 m RNA表达较假手术组明显降低(P<0.05),参附组较模型组升高(P<0.05)。结论参附注射液能减轻脓毒症大鼠的心肌损伤,其作用机制与上调Bcl-2 m RNA表达、减少心肌细胞凋亡相关。Objective： To investigate the anti-myocardial injury mechanism of Shengfu Injection by the change of myocardial enzyme and the expression of Bcl-2 and myocardial cell apoptosis in rat model with sepsis. Meth- ods： 24 male SD rats were randomly divided into three groups： the sham-operated group,the model group and the Shenfu group, 8 rats in each. Rat sepsis models were established with CLP. The change of myocardial enzyme spectrum was observed. Myocardial apoptosis was detected with TUNEL,and the expression of Bcl-2 mRNA in myocardial cell was detected with RT-PCR. Results： The rise of myocardial enzyme spectrum in Shengfu Injec- tion group was lower than that of the model group （P〈 0.05）. Myocardial enzyme level of the model group was higher than that of the sham-operated group （P〈 0.05）. TUNE L scores of cardiac muscle cells and MOD of Shen^u Injection group was lower than that of the model group （P〈 0.05）, and higher than that of the sham-oper- ated group （P〈0.05）. The expression level of Bcl-2 mRNA in myocardial cell of model group was lower than the sham-operated group （P〈 0.05）,while the Shengfu Injection group was higher than the model grou （P〈 0.05）. Conclusons： Shengfu Injection can alleviate injury in cardiac muscle caused by sepsis by upregulating the ex- pression level of BCL-2 and reducing myocardial apoptosis.

关 键 词：参附注射液 脓毒症 细胞凋亡 BCL-2 

分 类 号：R285.5[医药卫生—中药学]