芹菜素对大鼠脑缺血再灌注后的转化生长因子-β_1表达的影响  

Effects of Apigenin on the Expression of Transforming Growth Factor-β_1 after Cerebral Ischemia-reperfusion in Rats

作　　者：王果[1] 陈翔[2] 

机构地区：[1]浙江省杭州市第一人民医院,浙江杭州310000 [2]温州医科大学附属第二医院育英儿童医院康复中心,浙江温州325000

出　　处：《中国中医急症》2015年第9期1546-1548,1594,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省温州市科技局国际合作项目(H20070034)

摘　　要：目的探讨转化生长因子-β1(TGF-β1)在大鼠脑缺血再灌注后不同时间的表达变化及芹菜素对其表达的影响。方法采用改良线栓法建立大鼠脑缺血再灌注后不同时间损伤模型。随机分为假手术组、模型组、芹菜素组和地塞米松组,各手术组按再灌注时间不同又分为再灌注6 h组、24 h组、72 h组、7 d组4个亚组。各组麻醉清醒后均给予神经行为学评分,每组1只,大鼠予2,3,5-氯化三苯基四氮唑染色观察脑梗死灶,6只免疫组化法测定转化生长因子-β1的表达。结果在芹菜素72 h组神经行为学评分低于模型72 h组(P<0.05);各手术组均出现白色梗死灶;缺血再灌注后TGF-β1表达增加,于24、72 h达高峰,芹菜素组能显著提高TGF-β1的表达,与模型组比较差异显著(P<0.05)。结论芹菜素可增加脑缺血再灌注后TGF-β1的表达,提示芹菜素可能通过TGF-β1促进脑组织损伤的修复,达到神经损伤的保护作用。Objective： To investigate the effects of apigenin（APG） on the expression of transforming growth fac- tor-β1 （TGF-β1 ） of focal cerebral ischemia-reperfusion in rats. Methods： Cerebral ischemia was induced by middle cerebra artery occlusion. The rats were randomly divided into four groups including Sham-operated, Mode, APG and Dexamethasone groups. All the operated groups were divided into different groups ：reperfusion 6 h group, 24 h group,72 h group and 7 d group. Neurological behavior was assessed with five score methods. After anesthesia awake,each group was given neurological behavior scores. One brain slice was observed by 2,3,5- triphenyl tetrazolium chloride （TTC） stainning,and the expression of TGF-β1 in another six brain slices were measured by immunohi stochemistry. Results： Neurological behavior scores of the rats in A72 h group was signif- icant lower than those in ischemia M72 h group （P〈 0.05）. Typical cortical infarct lesions in Mode group were found by TYC stainning. The expression of transforming growth factor-β1 in normal brain tissue was in very low volume. The expression of transforming growth factor-β1 were enhanced immediately after ischemia,and reached to peak at 24 hour and 72 hour after ischemia respectively, Apigenin could significantly increase the expressions of TGF-β1 than ones at the corresponding time points in group M respectively （P〈 0.05）. Conclusion： Apigenin can increase the expression of transforming growth factor-β1 in transient focal cerebral ischemia and reperfusion in rats. They may repair ischemia brain tissue and improve neurological function recovery by transforming growth factor-β1 .

关 键 词：芹菜素 脑缺血再灌注 2 3 5-氯化三苯基四氮唑染色 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]