藏羊DQB1基因RT-PCR扩增及序列分析  

RT-PCR Amplification and Sequence Analysis of DQB1 Gene of Tibetan Sheep

作　　者：张晓芬[1] 冶贵生[1] 马玉花[1] 贺晓龙[1] 康明[1] 张爽[1] 韩志辉[1] 贾跃宁 张洪波[1] 

机构地区：[1]青海大学农牧学院,西宁810016

出　　处：《湖北农业科学》2015年第15期3784-3785,3790,共3页Hubei Agricultural Sciences

基　　金：青海省科技厅科研项目(2012-Z-930Q);青海大学"123高层次人才培养工程"项目

摘　　要：提取藏羊脾脏总RNA,设计DQB1基因引物并进行反转录,扩增产物测序后利用生物软件进行序列分析。结果表明,藏羊DQB1基因长度为786 bp,编码261个氨基酸。藏羊DQB1基因与参考美利奴羊DQB1基因、中国美利奴细毛羊MHCⅡ抗原基因、山羊DQB1基因的核苷酸序列同源性依次为95.8%、95.9%、95.3%,氨基酸序列同源性依次为91.2%、92.0%、91.2%。Total RNA from spleen of Tibetan sheep was extracted and reverse transcribed, and primers of DQB] gene were designed for PCR amplification. Moreover, the gene sequence was analyzed by DNAStar software after the PCR products was sequenced. The results showed that the length of DQB1 gene was 786 bp, encoding 261 amino acids, and compared with DQB1 gene of Merino sheep, MHC Ⅱ antigen gene of Merino Fine-Wool sheep and DQB1 gene of goat, the nucleotide sequence homology of Tibetan sheep were 95.8%, 95.9%, 95.3% respectively, and the amino acid sequence homology were 91.2%, 92.0%, 91.2% respectively.

关 键 词：藏羊 DQB1基因 RT-PCR 序列分析 

分 类 号：Q75[生物学—分子生物学] S826.83[农业科学—畜牧学]