疮灵液载入胶原促进慢性创面的愈合  

Chuanglingye-loaded collagen promotes chronic wound healing

作　　者：姚昶[1] 江虹[1] 许岩磊[1] 朱永康[1] 

机构地区：[1]南京中医药大学附属医院,江苏省南京市210029

出　　处：《中国组织工程研究》2015年第34期5518-5522,共5页Chinese Journal of Tissue Engineering Research

基　　金：江苏省自然科学基金(BK2010598);江苏省中医药领军人才项目(LJ2009002);江苏省高校优势学科建设项目Ⅱ期(012062003010);江苏省中医院高峰学术人才培养(y2014cr06)~~

摘　　要：背景:前期研究表明,疮灵液具有较好的抗炎作用,可抑制血小板聚集及血栓形成,促进伤口愈合。目的:观察将疮灵液载入胶原后对慢性创面愈合的影响。方法:在40只大鼠背部制作慢性创面模型,造模后3 d随机均分为4组,对照组以载0.2 mL生理盐水纱布外敷创面,疮灵液组以载0.2 mL疮灵液纱布外敷创面,胶原组以胶原海绵覆盖创面,疮灵液载入胶原组以载0.2 mL疮灵液胶原覆盖创面。造模时及造模后第3,7,15天测定创面面积,创面分泌物中白细胞总数、白细胞介素6与肿瘤坏死因子α水平,创面肉芽中羟脯氨酸、基质金属蛋白酶1,2,9的水平。结果与结论:疮灵液载入胶原组、疮灵液组造模后第3,7,15天肿瘤坏死因子α、基质金属蛋白酶1,2,9水平均低于对照组、胶原组(P<0.01,P<0.05),造模后第3,7,15天白细胞总数显著低于对照组、胶原组(P<0.01,P<0.05),前两组间各指标比较差异无显著性意义;疮灵液载入胶原组造模后第3,7,15天羟脯氨酸水平高于其余3组(P<0.05);疮灵液载入胶原组造模后15 d创面面积显著低于其余3组(P<0.01);造模后15 d,4组间白细胞介素6水平比较差异无显著性意义。表明疮灵液载入胶原能显著抑制慢性创面炎症细胞增殖与浸润,抑制创面炎症因子与基质金属蛋白酶分泌,促进慢性创面愈合。BACKGROUND：It has been shown thatChuanglingye has good anti-inflammatory effects, inhibits platelet aggregation and thrombus and improve wound healing. OBJECTIVE：To investigate the effects ofChuanglingye-loaded colagen on regulating inflammation and healing process of chronic wounds. METHODS： Forty rats were selected to make chronic wound models and randomly divided into four groups at 3 days after modeling： control group, colagen group,Chuanglingye group,Chuanglingye-loading colagen group, 10 rats in each group, respectively treated with 0.2 mL normal saline, 0.2 mL Chuanglingye, colagen and Chuanglingye-loaded colagen. The size of wounds was measured when modeling and at 3, 7 and 15 days after modeling. Amount of white blood cels, interleukin-6 level and tumor necrosis factor-α level in wound exudates were detected. The levels of hydroxyproline and matrix mentaloproteases （MMPs）, including MMP-1, MMP-2, MMP-9, in the granulation tissue, were also detected. RESULTS AND CONCLUSION：At 3, 7, 15 days after modeling, the amount of white blood cels and levels of interleukin-6, tumor necrosis factor-α, MMP-1, MMP-2, MMP-9 were significantly lower in the Chuanglingye-loaded colagen group andChuanglingye group compared to the control group and colagen group （P 〈0.01, P 〈 0.05）. But there was no significant difference betweenChuanglingye-loaded colagen group and 〈br〉 Chuanglingye group. The level of hydroxyproline in the granulation tissue was significantly higher in the Chuanglingye-loaded colagen group than the other three groups at 3, 7, 15 days after modeling （P 〈0.05）, and the size of wound in theChuanglingye-loaded colagen group was significantly lower than that in the other three groups at 15 days after modeling （P 〈0.01）. In addition, no significant difference was found among the four groups at 15 days after modeling. These findings indicate thatChuanglingye-loaded colagen can significantly depress proliferation and infiltration of inflammatory cels on the wound surface, dec

关 键 词：生物材料 材料相容性 疮灵液 胶原 慢性创面 炎症 创面愈合 江苏省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]