影响黑萨福克绵羊体内原核胚生产的因素  

Influencing Factors of Pronuclear Embryos Formation in vivo Black Suffolk Sheep

作　　者：柴孟龙[1,2,3] 魏巍[4] 何长久[2] 王军[1] 卫喜明[3] 张洪涛[3] 陈洋[1] 刘大森[4] 吕文发[1] 刘国世[2] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]中国农业大学动物科技学院,北京100193 [3]黑龙江省农垦科学院畜牧兽医研究所,黑龙江哈尔滨150090 [4]东北农业大学动物科技学院,黑龙江哈尔滨150030

出　　处：《黑龙江动物繁殖》2015年第5期6-10,共5页Heilongjiang journal of animal reproduction

基　　金：国家科技支撑项目(2011BAD19B02-4);转基因重大专项(2014ZX08008-002B;2011ZX08008-005)

摘　　要：为提高黑萨福克绵羊原核期胚胎的生产效率,通过CIDR＋FSH＋PMSG和CIDR＋FSH＋PG两种激素方案对供体进行超数排卵,将冲出的胚胎移植入同期发情处理的受体。其中CIDR＋FSH＋PMSG注射组供体平均黄体数、可用胚数和移植妊娠率均高于CIDR＋FSH＋PG注射组（P〈0.05）。同时,在CIDR＋FSH＋PMSG注射组,不同FSH剂量注射对黑萨福克绵羊超数排卵有影响。在180-240 IU剂量组,供体羊的平均黄体数、可用胚胎数高于另一组。繁殖季节（秋季）供体平均黄体数、可用胚胎数均显著高于春季（P〈0.05）。The objective of this study was to improve the efficiency of Black Suffolk pronuclear -stage embryo yield to be used for DNA microinjection in a transgenesis program.The Black Suffolk sheep was superovulated by CIDR ＋PMSG ＋FSH and CIDR ＋FSH ＋PG. Then the flushed out embryo was transferred to the estrus synchronized recipient by speculum insemination＋nature mating.In the CIDR＋FSH＋PMSG injection group, the average number of corpus luteum, available embryo and pregnancy rate from the Black Suffolk were significantly higher compared to the CIDR＋FSH＋PG injection group （ P〈0.05 ） . Meanwhile, different dose of FSH injection had a impact on the Black Suffolk sheep superovulation.When the FSH injection dose was during 180 -240 IU, the average number of corpus luteum and available embryo from the Black Suffolk sheep were 15.87 and 13.88, respectively.The average number of corpus luteum and available embryo were significantly higher in the breeding season （ autumn） compared to spring（ P〈0.05） .

关 键 词：黑萨福克 超数排卵 原核胚 

分 类 号：S826.3[农业科学—畜牧学]