超声波辅助花粉介导法获得转BtCry1Ac基因玉米的研究  

Research on BtCry1Ac Transgenic Maize Lines Using Ultrasonic-assisted Pollen Mediated Plant Transformation Method

作　　者：南芝润[1] 惠国强[1] 罗绮[1] 宋红霞[2] 吴家和[3] 孙毅[4] 张红梅[1] 

机构地区：[1]山西省农业科学院玉米研究所,山西忻州034000 [2]山西大学生物工程学院,山西太原030031 [3]中国科学院微生物研究所,北京100101 [4]山西省农业科学院生物技术研究中心,山西太原030031

出　　处：《黑龙江农业科学》2015年第9期22-25,共4页Heilongjiang Agricultural Sciences

基　　金：国家农业部转基因专项资助项目(2008ZX08003-001)

摘　　要：为了进一步分析玉米基因功能及基因转化,并为玉米抗虫提供种质资源,利用超声波辅助花粉介导法,将抗虫基因Cry1Ac导入玉米优良自交系昌7-2中,获得大量的转基因植株。结果表明:转基因植株经过草铵膦抗性筛选、PCR检测、BT蛋白试纸条检测和室内抗虫性鉴定等分析,最后获得高抗玉米螟的转BtCry1Ac基因株系9个。培育出了高抗玉米螟的种质资源材料,同时也建立了超声波辅助花粉介导转化玉米的高效转化体系,该方法是简捷、快速有效、无基因型限制的植物转化方法。In order to further analyze the gene functions and transformation of maize ,and provide germplasm re‐sources for maize insect‐resistant .The insect resistant Cry1Ac gene was used to transfer into maize inbred lines Chang 7‐2 ,through the method of ultrasonic‐assisted pollen mediated transformation ,more transgenic‐maize plantlets were obtained .The results showed that the transgenic plants were detected by PCR detection , Bt protein strip and raising insect assay in laboratory .Nine elite transgenic lines with high resistance to Ostrinia nubilalis were developed .In conclusion ,it not only provided germplasm resources for maize beeding but also es‐tablished the ultrasonic processing pollen mediated maize genetic transformation method ,which was a simple , rapid and effective method for the transformation of plants .

关 键 词：玉米 CRY1AC基因 超声波处理花粉介导法 抗虫性 

分 类 号：S513[农业科学—作物学]