豚鼠、大鼠和小鼠内淋巴囊组织学的差异  

The Anatomic Differences of Endolymphatic Sac among Common Laboratory Rodents

作　　者：丁大雄 杨风波[1,2,3] 吕萍[3] 黄国威[1,2,3] 王晓东[1,2] 刘晨[1,2] 张悦[1,2] 杨仕明[1,2] 于宁[1,2] 

机构地区：[1]解放军总医院耳鼻咽喉头颈外科 [2]中国人民解放军总医院耳鼻咽喉研究所,北京100853 [3]川北医学院附院耳鼻咽喉科

出　　处：《听力学及言语疾病杂志》2015年第5期497-499,共3页Journal of Audiology and Speech Pathology

基　　金：国家973计划重大科学研究计划干细胞项目(2012CB967900);国家自然基金项目(81470700,81241034);总后军用标准项目(BWS14B080);总装项目(JDZYY20132);解放军总医院苗圃基金(12KMM07);南充市研发资金项目(14A0084);四川省卫生厅课题(110298);北京市项目(PXM2014-178304-000002-00130228)联合资助

摘　　要：目的比较豚鼠、大鼠和小鼠内淋巴囊组织学的差异,为内淋巴囊相关疾病的研究提供形态学基础。方法成年豚鼠、SD大鼠、昆明小鼠各5只,心脏灌流后,断头取颞骨水平位常规组织切片及HE染色观察三种动物的内淋巴囊形态特征。结果三种动物内淋巴囊都含有囊腔、上皮、上皮下及囊周组织;豚鼠内淋巴囊中部上皮存在由大量细胞形成的乳头和褶皱,且乳头上可见胞质深染体积较大的细胞突向囊腔,而大鼠和小鼠内淋巴囊中部上皮仅见到胞质深染体积较大的细胞突向囊腔,少见或未见到乳头或褶皱形成。结论豚鼠与大鼠、小鼠的内淋巴囊组织形态学差异较为明显,而大鼠、小鼠间内淋巴囊形态差异较小。Objective To compare the differences of laboratory rodents endolymphatic sac （ES） in histology , and to provide an anatomic basis for the clinical research of ES -related diseases .Methods The temporal bones of guinea pigs ,SD rats and KM mice were fixed by heart perfusion ,sectioned by paraffin at the horizontal position and stained by hematoxylin -eosin （HE） .Results All 3 rodents ES contained sac ,epithelium ,sub-mucosal tissue and saccal stroma .The cells were bulky ,stained deep and protruded into the cavity to form a large number of nipples and wrinkles in the middle ES epithelial of guinea pigs .But the nipples or wrinkles was rarely observed in the middle ES epithelial in rat and mouse .Conclusion The histology of ES has significant difference among guinea pig ,mouse and rat .While the difference betweeen mouse and rat is not obvious .

关 键 词：啮齿类动物 耳蜗 内淋巴囊 

分 类 号：R322.923[医药卫生—人体解剖和组织胚胎学]