豚鼠耳蜗Hensen细胞脂滴的性质与分布  

The Distribution of the Lipid Droplets within Hensen Cells in the Guinea-pig Cochlea

作　　者：杨风波[1] 丁大雄 吕萍[1] 程晓婷[2] 任红苗[3] 黄国威[1] 王晓东[1] 刘晨[1] 张悦[4,5] 丛涛[4,5] 杨仕明[4,5] 翟所强[4,5] 于宁[4,5] 

机构地区：[1]川北医学院附院耳鼻咽喉科,南充637000 [2]福建医科大学附属第一医院耳鼻咽喉头颈外科 [3]中南大学湘雅二医院耳鼻咽喉科 [4]解放军总医院耳鼻咽喉头颈外科 [5]中国人民解放军总医院耳鼻咽喉研究所

出　　处：《听力学及言语疾病杂志》2015年第5期500-504,共5页Journal of Audiology and Speech Pathology

基　　金：国家973计划重大科学研究计划干细胞项目(2012CB967900与2014CB943002);军队项目(BWS14B080;JDZYY20132);国家自然基金项目(81271081与81470700);四川省卫生厅课题(110298);北京市项目(PXM2014-178304-000002-00130227)联合资助

摘　　要：目的探索豚鼠内耳Hensen细胞(Hensen cells,HC)中透明物质的性质及分布。方法 10只豚鼠分离制备好耳蜗单离Hensen细胞后,采用中性脂滴的特异分子探针Bodipy 493/503、苏丹Ⅲ、油红O染色及锇酸再固定四种染色方法对内耳组织进行染色,观察耳蜗不同区域Hensen细胞中脂滴的分布情况。结果豚鼠耳蜗不同区域Hensen细胞内均存在高透亮物质,从顶回至底回其体积逐渐变小,数量逐渐增加,至底回逐渐消失;该物质经Bodipy 493/503分子探针特异标记为绿色,被苏丹Ⅲ染色为橘红色,被油红O染色为宝石红,被锇酸后固定后呈黑色颗粒,证实为脂滴。结论四种染色方法对内耳组织的染色结果均提示内耳Hensen细胞中透明物质为脂类物质。Objective To study the distribution and properly of the transparent globules within Hensen cells （HC） of guinea -pig Corti organ .Methods The cochlear epithelial cells were isolated from 10 guinea pigs .The cells of cochlea were marked by Bodipy493/503 ,sudan III ,oil red O ,and osmium tetroxide .Results The transpar‐ent globules within the HCs of the guinea -pigs were green staining by Bodipy493/503 ,jacinth staining by Sudan III ,ruby red by oil red O .And they were black globules stripe as post -fixed in 1% osmium tetroxide .Conclusion The results indicate that the transparent globules within guinea -pigs HCs＇lipid droplets by four methods .

关 键 词：豚鼠 耳蜗 Hensen细胞 脂滴 

分 类 号：R339.16[医药卫生—人体生理学]