雷帕霉素及环孢素对人类外周血CD_4^+CD_(25)^+调节性T细胞体外增殖的影响  

Effect of rapamycin and ciclosporin on proliferation of human periheral blood CD_4^+CD_(25)^+ regulatory T cells

作　　者：赵冰清[1] 朱志军[2] 王永磊[3] 

机构地区：[1]天津市第二人民医院,天津300192 [2]首都医科大学附属北京友谊医院 [3]天津医院

出　　处：《山东医药》2015年第34期19-21,共3页Shandong Medical Journal

摘　　要：目的观察雷帕霉素与环孢素对人类外周血CD+4CD+25调节性T细胞(Treg)体外增殖的影响。方法利用免疫磁珠法提取健康成人外周血CD+4CD+25Treg,流式细胞仪检测CD+4CD+25Treg纯度;将羧基荧光素乙酰乙酸进行标记的CD+4CD+25Treg分为A、B、C组,分别加入100 nmol/L雷帕霉素、410 nmol/L环孢素及空白培养基;培养7 d后上流式细胞仪,计算原代细胞所占比例。结果 A组原代细胞所占比例为8.8%±3.2%,B组为48.9%±7.0%,C组为33.2%±4.3%;A、B组分别与C组比较,P均<0.05。结论雷帕霉素可促进CD+4CD+25Treg的体外增殖,有利于移植后患者免疫耐受的形成;而环孢素则抑制CD+4CD+25Treg的体外增殖,不利于免疫耐受的形成。Objective To observe the influence of rapamycin and ciclosporin on the proliferation of human periheral blood CD4＋CD2＋5 regulatory T cells.Methods The CD4＋CD2＋5 regulatory T cells （ Treg cells） from human peripheral blood were isolated by immunomagnetic bead separation, the purity of CD4＋CD2＋5 Treg was measured by flow cytometry.We divid-ed the CD4＋CD2＋5 Treg into the groups A, B and C, which were added with 100 nmol/L rapamycin, 410 nmol/L cyclospo-rine and the blank culture medium.After 7 days′culture, we calculated the percentage of primary Treg cells of different groups by flow cytometry.Results The percentage of primary Treg cells in the groups A, B and C were 8.8%±3.2%, 48.9%±7.0%and 33.2%±4.3%, respectively.Significant difference was found when we compared groups A and B with group C, respectively （ all P 〈0.05）.Conclusion Rapamycin can significantly promote the proliferation of CD4＋CD2＋5 Treg in vitro, and it promotes the formation of immune tolerance in patients after transplantation;but cyclosporine inhibits the proliferation of CD4＋CD2＋5 Treg and hampers the progression.

关 键 词：调节性T细胞 雷帕霉素 环孢素 免疫耐受 细胞培养 

分 类 号：R318[医药卫生—生物医学工程]