SPECT/MRI双模态显像剂SPION-DMSA-^(99)Tc^m的制备及其性质研究  

Preparation and Evaluation of SPION-DMSA-^(99)Tc^m as SPECT/MRI Dual-modal Imaging Agent

作　　者：王晓[1,2] 崔海平[1,2] 史旭东[1,2] 梁积新[1] 孙钰林 申一鸣[1,2] 沈浪涛[1,2] 

机构地区：[1]中国原子能科学研究院国家同位素工程技术研究中心,北京102413 [2]原子高科股份有限公司,北京102413

出　　处：《原子能科学技术》2015年第9期1557-1564,共8页Atomic Energy Science and Technology

摘　　要：为制备纳米材料SPION-DMSA及其标记物SPION-DMSA-99Tcm,探讨该标记物作为SPECT/MRI双模态显像剂的可能性,本工作先采用高温热解法合成了SPION,然后用DMSA包覆获得SPIONDMSA并进行各种表征。用99Tcm标记SPION-DMSA得到SPION-DMSA-99Tcm,并对该标记物进行荷U87MG人脑神经胶质瘤裸鼠的生物分布和显像研究。实验结果表明,SPION-DMSA具有超顺磁性,99Tcm标记率大于98%。SPION-DMSA-99Tcm在血液中清除较快,在肝脏中的摄取较高。SPIONDMSA-99Tcm与DMSA-99Tcm(Ⅴ)在小鼠体内的生物分布差异较大。SPION-DMSA-99Tcm在肿瘤中并没有明显的摄取。SPION-DMSA和SPION-DMSA-99Tcm的MRI和SPECT显像结果表明,SPIONDMSA和SPION-DMSA-99Tcm的肿瘤被动靶向作用有限。因此,对于荷U87MG人脑神经胶质瘤裸鼠,SPION-DMSA-99Tcm还不是一种理想的SPECT/MRI双模态显像剂。Nanoparticle SPION‐DMSA and its radiolabeled SPION‐DMSA‐99 Tcm were prepared in order to investigate the possibility of SPION‐DMSA‐99 Tcm as a SPECT /MRI dual‐modal imaging agent .SPION was synthesized by a hydrothermal process ,and then coated with DMSA to afford SPION‐DMSA .SPION‐DMSA was characterized by means of various methods .SPION‐DMSA‐99 Tcm was obtained by labeling with 99 Tcm . The data of biodistribution and the SPECT and MRI images were acquired after injecting SPION‐DMSA‐99 Tcm into the nude mice bearing U87MG human glioma in various inter‐vals .The results show that SPION‐DMSA exhibits super‐magnetic properties . The＆nbsp;labeling yield of SPION‐DMSA with 99 Tcm was more than 98% .SPION‐DMSA‐99 Tcm is cleared from the blood quickly and has higher uptake in the liver . There was a big difference of biodistributions between SPION‐DMSA‐99 Tcm and DMSA‐99 Tcm （ Ⅴ ） in the nude mice .The uptake of SPION‐DMSA‐99 Tcm in tumor is not obvious .The results of imaging made by MRI and SPECT using SPION‐DMSA and SPION‐DMSA‐99 Tcm respectively show that the tumor passive targeting of SPION‐DMSA and SPION‐DMSA‐99 Tcm is limited . Therefore , SPION‐DMSA‐99 Tcm is not an ideal SPECT /MRI dual‐modal imaging agent for the nude mice bearing U87MG human glioma .

关 键 词：超顺磁氧化铁纳米颗粒 二巯基丁二酸 ^99TC^M SPECT/MRI双模态显像剂 

分 类 号：O615.4[理学—无机化学]