复脉汤预处理对心肌缺血诱导心律失常大鼠心肌细胞蛋白激酶C与Cx43磷酸化信号通路的影响  

Study the Effect of Fumai Decoction Pretreatment on PKC and P-Cx43 S368 in Myocardial Ischemia Induced Arrhythmia Rat

作　　者：龚一萍[1] 程臻 陈盈[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]东阳市中医院,浙江金华322110

出　　处：《云南中医学院学报》2015年第4期15-18,22,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：浙江省自然科学基金(LY12H27010)

摘　　要：目的探讨复脉汤对心律失常大鼠心肌细胞蛋白激酶C与连接蛋白43(Cx43)磷酸化信号通路的影响。方法将实验大鼠分为假手术组、模型组、酒石酸美托洛尔组(104 mg·kg-1)、炙甘草汤组(9.40 g·kg-1)、复脉汤低剂量组(9.38 g·kg-1)、复脉汤中剂量组(18.75 g·kg-1)、复脉汤高剂量组(37.50g·kg-1),共7组。采用结扎冠状动脉左前降支1 h建立心肌缺血模型,通过复脉汤预处理对其进行干预,运用蛋白免疫印迹法(Western blot)检测蛋白激酶C(PKC)和心肌细胞缝隙连接蛋白43总量(Total-Cx43)及其Ser368位点磷酸化(P-Cx43 S368)蛋白水平的表达情况。结果与假手术组相比,模型组PKC与Total-Cx43以及P-Cx43 S368表达均显著降低(P<0.01);与模型组相比,复脉汤中、高剂量组、酒石酸美托洛尔组PKC与Total-Cx43以及P-Cx43 S368表达上调,均表现出显著性差异(P<0.01);炙甘草汤组与复脉汤低剂量组也能促进PKC与Total-Cx43以及P-Cx43 S368表达,与模型组相比具有统计学差异(P<0.05)。结论复脉汤通过上调心肌缺血大鼠心肌PKC与Total-Cx43以及P-Cx43 S368表达,增强缝隙连接(GJ)通道的传导性和通透性,减轻其心肌损伤程度,这可能是复脉汤对缺血损伤大鼠心律失常的保护效应机制之一。Objective To investigate the effect of Fumai decoction on PKC and P-Cx43 $368 in arrhythmia rat. Methods Divided the rats into sham operation group, model group, metoprolol group （104mg·kg^-1）, group of Zhigancao decoction, low dose, middle dose and high dose groups of Fumai decoction. Rats of myocardial ischemia model were induced by left anterior descending ligation for one hour.After the treatment of Fumai decoction, the PKC, Total-Cx43 and P-Cx43 S368 expression was characterized by Westernblot. Results Compared with sham operation group, all the PKC, Total-Cx43 and P-Cx43 S368 expression of model group were significant reduced （P〈0.01）. Compared with model group, all the PKC, Total-Cx43 and P-Cx43 S368 expression of middle dose and high dose groups of Fumai decoction and metoprolol group were significant increased （P〈0.01）. PKC, Total-Cx43 and P-Cx43 S368 of group of Zhigancao decoction and low dose group of Fumai decoction also expressed higher than model group （P〈0.05）. Conclusion Fumai decoction can increase the PKC, Total-Cx43 and P-Cx43 S368 expression in myocardial ischemia rats, increase the gap junction channel permeability and conductance, and decrease the level of myocardial injury. This may be one of the mechanism of Fumai decoction to treat arrhythmia in myocardial ischemia rats.

关 键 词：复脉汤 心律失常 信号通路 Total-Cx43 P-Cx43 S368 

分 类 号：R285.5[医药卫生—中药学]