微丝附着梁蛋白在大鼠自体移植静脉中的表达变化及其与内膜增生的关系  

Expression change of girders of actin filament in autologous vein graft of rat and its relationship with intimal hyperplasia

作　　者：梁路东 黄强信[1] 蓝志存 霍天明[1] 李世康[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁市530021

出　　处：《广西医学》2015年第7期895-899,共5页Guangxi Medical Journal

基　　金：广西教育厅科研项目(2012MS017);广西科学研究与技术开放计划项目(桂科攻1355005-4-8)

摘　　要：目的探讨微丝附着梁蛋白(Girdin)在大鼠自体移植静脉中的表达变化及其与内膜增生的关系。方法 28只雄性SD大鼠随机均分为4组,建立颈外静脉移植至颈总动脉模型,于术后3 d、7 d、14 d及28 d采集移植静脉,同时取对侧静脉作对照。光镜下观察血管内膜,应用图像软件计算新生内膜增生厚度,免疫组化及Western Blot法检测Girdin及增殖细胞核抗原(PCNA)蛋白在静脉桥及对侧静脉血管壁的表达变化情况。结果移植术后,移植静脉内膜术后7 d增厚明显(P<0.01),14 d时增厚更为明显,28 d时达到高峰;免疫组化结果显示Girdin和PCNA蛋白均在术后3 d明显增加(P<0.01),前者于14 d出现高峰,后者于7 d达高峰,而后逐渐下降;术后Girdin蛋白表达与PCNA蛋白表达呈正相关(r=0.938,P<0.01)。对侧静脉有少量Girdin蛋白表达,而几乎无PCNA蛋白表达。结论静脉桥术后Girdin蛋白的表达增高,并与静脉桥平滑肌细胞的增殖密切相关,这可能在静脉桥术后内膜增生中起重要作用。Objective To explore the expression change of girders of actin filaments（ Girdin） in autologous vein graft of rat and its relationship with intimal hyperplasia.Methods A total of 28 male Sprague-Dawley（SD） rats were randomly divided into four groups,and the external jugular vein-to-common carotid artery transplant model was established.The vein graft samples were harvested 3,7,14 and 28 days after operation,respectively,and contralateral veins were collected as control.Intima was observed under the optical microscope and neointima thickness was calculated using the computer image analysis system.The expression changes of Girdin and proliferating cell nuclear antigen（ PCNA） in the vessel wall of grafted veins and contralateral veins were detected by both immunohistochemistry and Western Blot.Results In the grafted veins,intima was significantly thicker on the 7th day after operation （P〈0.01）,and this change was more significant on the 14th day,then culminated on the 28th day.The result of immunohistochemistry showed that both Girdin and PCNA expressions increased obviously on the third day after operation （P〈0.01）,peaked on the 14th（Girdin） or 7th（PCNA） day,and then decreased gradually after that.A positive correlation existed between Girdin and PCNA expressions postoperatively（r=0.938,P〈0.01）.A little expression of Girdin and little expression of PCNA were found in the contralateral veins.Conclusion Girdin expression increases after vein grafting,and is closely related to the proliferation of media smooth muscle cells in grafted veins,which might play an important role in the process of intimal hyperplasia after vein grafting.

关 键 词：自体静脉移植 微丝附着梁蛋白 细胞增殖核抗原 内膜增生 大鼠 

分 类 号：R622.4[医药卫生—整形外科]