RP-HPLC法测定小儿清肺止咳片中栀子苷的含量  

Determination of Geniposide in Xiao'er Qingfei Zhike Tablets by RP-HPLC

作　　者：朱晓静[1] 朱文娟[1] 宋粉云[1] 钟兆健[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《海峡药学》2015年第8期63-65,共3页Strait Pharmaceutical Journal

基　　金：广东省自然科学基金资助项目(No.5002841)

摘　　要：目的：建立测定小儿清肺止咳片中栀子苷含量的方法。方法采用反相高效液相色谱法，Diamonsil－C18柱，乙腈－水（12∶88）为流动相，流速为1．0mL·min －1，检测波长为240nm，柱温为30℃。结果栀子苷浓度在5．0～50．0μg？ mL －1间具有良好的线性关系（r＝0．9998），栀子苷平均回收率为100．4％，方法精密度（RSD）为0．44％（n＝6）。结论该法可用于小儿清肺止咳片中栀子苷的含量测定。OBJECTIVE To establish the RP-HPLC method for determination of geniposide in Xiaoer Qingfei Zhike Pian.METHODS A RP-HPLC method was established.The chromatographic column was Diamonsil-C18 .The mobile phase was a mixture of acetonitrile–water（12∶88,V/V）.The flow rate was 1.0mL·min-1 ,and the detec-tion wavelength was 240 nm.RESULTS The linear range of geniposide was 5.0~50.0μg？ mL-1 （ r=0.9998 ） .The average recovery for geniposide was 100.4%, precision of the method was 0.44%（ RSD, n =6 ） .CONCLUSION The method can be used for quantitative determination of the preparation.

关 键 词：小儿清肺止咳片 栀子苷 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]