TNF-α对小鼠骨骼肌成肌细胞生理功能的影响  

Effects of TNF-α on murine skeletal muscle myoblasts

作　　者：刘文斌[1] 吴丽姿 肖坚[1] 

机构地区：[1]武汉轻工大学医学技术与护理学院,湖北武汉430023 [2]佛罗里达大学医学院,佛罗里达甘城fl32610

出　　处：《武汉轻工大学学报》2015年第3期31-36,共6页Journal of Wuhan Polytechnic University

摘　　要：肿瘤坏死因子(TNF-α)是一种炎症相关的细胞因子。通过基因组PCR对Maml1基因敲除和MAML1转基因小鼠进行了鉴定,通过去除血清和增加TNF-α来检测小鼠骨骼肌成肌细胞的抗饥饿能力。通过分化培养基诱导、TNF或staurosporine处理来研究成肌细胞肌管形成能力。结果表明,TNF-α促进了成肌细胞抗饥饿的能力,促进成肌细胞分化形成更多、更长、更持久的肌管。同样,MAML1基因也能够促进肌管形成。对进一步了解TNF-α对骨骼肌成肌细胞生理功能的影响提供了借鉴。Tumor necrosis factor,TNF,is a kind of cytokine related to inflammation. The genome PCRs were performed to identify the genotypes of Maml1-knock-out and MAML1-transgenic mice. The cellular anti-starvation ability was detected by removing serum and adding TNF-α,and myotube formation ability was detected by exchanging with differentiation medium and adding TNF or staurosporine. The results indicate that TNF-α helps murine skeletal muscle myoblasts the ability of anti-starvation and promotes their differentiation to form more myotubes and keeps them longer and more persistent. MAML1 promotes the differentiation of myoblasts as well. This study helps people to further understand the effects of TNF-α on the physiological functions of myoblasts.

关 键 词：肿瘤坏死因子-Α 成肌细胞 抗饥饿 细胞分化 肌管 

分 类 号：Q2[生物学—细胞生物学]