基于SRAP和TRAP标记的河北野生狗牙根种质遗传多样性分析  

Genetic Diversity Analysis of Wild Bermudagrass( Cynodon dactylon)Using SRAP and TRAP Markers in Hebei Province

作　　者：李会彬[1] 赵玉靖[1] 梁中银[1] 郭丽果[1] 王丽宏[1] 边秀举[1] 

机构地区：[1]河北省作物生长调控重点实验室/河北省作物种质资源实验室/河北农业大学,保定071001

出　　处：《植物遗传资源学报》2015年第5期1013-1019,共7页Journal of Plant Genetic Resources

基　　金：国家星火计划项目(2011GA1050003);河北农业大学科研发展基金项目

摘　　要：以SRAP和TRAP 2种标记技术对36份狗牙根材料的遗传多样性及亲缘关系进行了分析,其中包含34份河北省野生狗牙根种质资源。分别由238对SRAP和85对TRAP引物组合中筛选获得具有多态性的SRAP和TRAP引物组合各10对,PCR扩增总条带分别为186和161条,多态性条带156和132条,平均每对引物扩增出多态性条带各15.6和13.2条,多态性位点比率分别为83.4%和81.0%。2种标记合并进行聚类分析,所有供试的36份狗牙根材料遗传相似系数GS=0.519~0.983,平均为0.7。当GS=0.68时,可将36份供试材料分为4个类群。本研究结果表明河北野生狗牙根种质资源存在较丰富的遗传多样性,可为种质资源保护和选育优良狗牙根新品种提供科学依据。SRAP and TRAP markers were employed to investigate the genetic diversity and relationships of 36 bermudagrass（ Cynodon dactylon）accessions, including 34 wild bermudagrass germplasm resources of Hebei prov- ince. Ten primer pairs from 238 primers pairs of SRAP and ten primer pairs from 85 primers pairs of TRAP were se- lected and used for the present study. Ten primers pairs of SRAP and TRAP produced a total of 186 and 161 bands, including 156 and 132 polymorphic bands, averaged 15.6 and 13.2 polymorphic bands per primer pair, and the per- centages of polymorphic sites were 83.4% and 81.0% in average,respectively. Combined the both markers with cluster analysis, the genetic similarity among all accessions ranged from 0.519 to 0. 983 and with an average of 0. 7. When the GS was 0. 68, the 36 accessions were classified into four distinct clusters. The results indicated that there was substantial genetic diversity among the Cynodon dactylon germplasms in Hebei province, which provided a sci- entific basis for germplasm resources conservation and breeding fine cultivars of bermudagrass

关 键 词：狗牙根 种质资源 SRAP标记 TRAP标记 遗传多样性 

分 类 号：S543.9[农业科学—作物学]