腰椎间盘退变与局部转化生长因子β1及炎性细胞因子的关系  

Lumbar disc degeneration is associated with local transforming growth factor beta1 and inflammatory cytokines

作　　者：胡家美[1] 徐新华[1] 乐敏莉[2] 

机构地区：[1]湖北民族学院附属民大医院骨外科,湖北省恩施州445000 [2]湖北民族学院附属民大医院放射科,湖北省恩施州445000

出　　处：《中国组织工程研究》2015年第33期5318-5321,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来随着椎间盘退变分子水平研究的不断进展,转化生长因子β1基因在椎间盘细胞的增殖分化过程中具有一定作用,且参与了椎间盘的损伤修复过程,但转化生长因子β1是否也参与了椎间盘退变的病理生理过程目前尚无定论。目的:探讨腰椎间盘退变程度与转化生长因子β1及炎性细胞因子之间的关系。方法:选择72例椎间盘退变患者作为观察组(轻度22例,中度26例,重度24例),30例非椎间盘退变患者作为对照组,检测两组患者椎间盘局部转化生长因子β1及白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α等炎性细胞因子水平,在两组之间及不同椎间盘退变程度患者之间进行对比分析。同时采用直线相关分析法分析转化生长因子β1与炎性细胞因子及腰椎间盘退变的相关性。结果与结论:观察组患者腰椎间盘局部转化生长因子β1及白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α等炎性细胞因子水平均显著高于对照组(P<0.01)。重度退变患者腰椎间盘局部转化生长因子β1及白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α等炎性细胞因子水平显著高于轻度及中度患者(P<0.01),同时中度患者显著高于轻度患者(P<0.01)。转化生长因子β1与白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α等炎性细胞因子以及椎间盘退变程度均呈显著正相关(r=0.198,0.312,0.356,0.275,0.724,P<0.01)。提示转化生长因子β1及白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α等炎性细胞因子在退变椎间盘局部水平增高,且增高程度随着退变严重程度的增加而增加。BACKGROUND： With the research development of disc degeneration at molecular level, transforming growth factor β1 gene has a role in the proliferation and differentiation of intervertebral disc cells, and it is involved in the repair of intervertebral disc injury. But whether transforming growth factor β1 is also involved in pathophysiological process of disc degeneration has not been determined. OBJECTIVE： To discuss the relationship between transforming growth factor β1, inflammatory cytokines and degree of lumbar disc degeneration. METHODS： Twenty-two patients with lumbar disc degeneration （22 mild, 26 moderate and 24 severe cases） were enrolled as observation group, and another 30 patients without lumbar disc degeneration served as control group. Levels of transforming growth factor β1, interleukin-6, interleukin-8, tumor necrosis factor a were detected and compared among patients with mild, moderate and severe lumbar disc degeneration as well as between the observation and controlgroups. RESULTS AND CONCLUSION： Levels of transforming growth factor β1, interleukin-6, interleukin-8, tumor necrosis factor a were significantly higher in the observation group than the control group （P 〈 0.01 ）. In the observation group, the above-mentioned indicators were ranked as follows： severe cases 〉 moderate cases 〉 mild cases （P 〈 0.01 ）. There was a positive correlation between these indicators and the severity of lumbar disc degeneration （t=0.198, 0.312, 0.356, 0.275, 0.724, P 〈 0.01）. These findings indicate that the levels of transforming growth factor β1, interleukin-6, interleukin-8 and tumor necrosis factor a are increased with the severity of lumbar disc degeneration.

关 键 词：腰椎 椎间盘退化 转化生长因子Β1 白细胞介素1Β 组织构建 骨组织工程 腰椎间盘 退变 炎性细胞因子 

分 类 号：R318[医药卫生—生物医学工程]