一种辣椒轻斑驳病毒和辣椒斑驳病毒复合侵染的线椒病毒病害分子鉴定  

Molecular Identification of A Pepper Virus Disease Showing Mixed Infection of PepMoV with PMMoV

作　　者：鲁宇文[1] 彭杰军[1] 郑红英[1] 林林[1] 燕飞[1] 陈剑平[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,杭州310021

出　　处：《科技通报》2015年第9期85-88,共4页Bulletin of Science and Technology

基　　金：农业部公益性行业(农业)科研专项(201303028);浙江省农科院科技创新能力提升工程项目资助

摘　　要：上海奉贤区发生的线椒病害其症状表现为斑驳、花叶,且果实扭曲畸形,严重影响了辣椒的产量和品质.通过电镜病毒粒子观察和RT-PCR检测,确定其毒源为辣椒斑驳病毒（pepper mottle virus,PepMoV）和辣椒轻斑驳病毒（pepper mild mottle virus,PMMoV）复合侵染.根据它们的外壳蛋白（coatprotein,CP）核苷酸序列,在GenBank 上进行BLAST 比对,其同源性PepMoV 为85%-99%,PMMoV 为94%-99%.系统进化分析表明,PepMoV和PMMoV上海分离物变异均存在一定的地域相关性.这是首次在我国辣椒田间病毒病样中检测到这两种病毒的复合侵染.Peppers from Fengxian district in Shanghai showed symptoms of mottle, mosaic on leaves and distortion on fruits, which greatly reduced the yield and quality of pepper. The results of electronic microscope observation and RT-PCR indicated the mixed infection of pepper mottle virus with pepper mild mottle virus. Compared their complete coat protein nucleotides equences on GenBank suggested the homology of PepMoV-SH is about 85%~99% and 94%~99% to PMMoV-SH. Phylogenetic analysis showed the evolutions of PMMoV-SH and PepMoV-SH are related to their regional distribution. This is the first report in China about mixed infection of Pepper mild mottle virus with Pepper mottle virus in field.

关 键 词：辣椒斑驳病毒 辣椒轻斑驳病毒 复合侵染 序列分析 

分 类 号：S432.41[农业科学—植物病理学]