高效液相色谱检测水果蔬菜中噻苯隆的残留量  

HPLC Method for Detection of Thidiazuron Residues in Fruits and Vegetables

作　　者：黄玉南[1] 罗静[1] 乔成奎[1] 王超[1] 庞涛[1] 吴丰魁 成昕[3] 

机构地区：[1]中国农业科学院郑州果树研究所,农业部果品质量安全风险评估实验室(郑州),农业部果品及苗木质量监督检验测试中心(郑州),郑州450009 [2]赛默飞世尔科技(中国)有限公司,上海201206 [3]农业部农产品质量安全中心,北京100081

出　　处：《农药》2015年第9期664-666,677,共4页Agrochemicals

基　　金：中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2015-ZFRI);2015年国家农产品质量安全风险评估专项(GJFP201500202;GJFP201500302)

摘　　要：[目的]构建一种固相萃取-高效液相色谱仪测定水果蔬菜样品中噻苯隆残留量的方法。[方法]以梨、苹果、猕猴桃、圣女果、葡萄为基质,样品经过乙腈萃取,弗罗里硅土柱净化,再用高效液相色谱仪检测。[结果]噻苯隆在0.005-1.0 mg/L之间呈良好的线性关系,线性相关系数0.999 7。添加质量浓度在0.02、0.05、0.10 mg/L时,平均回收率在84.3%-104.7%之间,相对标准偏差为0.53%-4.01%。[结论]方法操作简单可靠,具有较高的准确度和灵敏度,可用于水果蔬菜中噻苯隆含量的测定。[Aims] The SPE-HPLC method was established to detect the thidiazuron residues in fruits and vegetables. [Methods] The thidiazuron residues in pear, apple, grape, kiwifruit and cherry tomato samples were extracted with acetonitrile, cleaned up by Florisil column and detected by high performance liquid chromatography （HPLC）. [Results] There was a good linearity when the concentrations ranged from 0.005 to 1.0 mg/L with the correlation coefficient of 0.999 7. When the addition levels were 0.02, 0.05, 0.10 mg/L, the recoveries of thidiazuron ranged from 84.3 to 104.7%, and the relative standard deviations were 0.53-4.01%. [Conclusions] The method is simple and easy, with good accuracy and sensitivity, which can be used for the detection of thidiazuron residues in fruits and vegetables.

关 键 词：噻苯隆 高效液相色谱法 水果 蔬菜 残留 

分 类 号：O657.72[理学—分析化学] TS255.7[理学—化学]