浙江1株水稻细菌性条斑病菌株的分离鉴定  

Isolation & Identification of a Bacterial Rice Leaf Streak Strain in Zhejiang Province

作　　者：路梅[1] 凌丹燕[1] 张发成 辛德东[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]金华市植保站,浙江金华321017

出　　处：《微生物学杂志》2015年第4期13-18,共6页Journal of Microbiology

基　　金：浙江省自然科学基金项目(Y3110194)

摘　　要：细菌性条斑病(简称细条病)是水稻的重要病害之一,随着气候的变暖,某些水稻品种有逐年发生加重的趋势,对产量影响较大。2013年浙江省金华市部分水稻种植区域发生细条病,发病严重的田块减产30%以上。采用组织分离法从发病水稻叶片分离获得6株细菌菌株,选择典型菌株JH01回接水稻幼苗,进行柯赫法则验证。接种后发病症状与自然发病症状一致,并重新分离得到此菌株,证明菌株JH01为水稻细条病的致病菌。通过形态学观察、常规生理生化指标测定、16S r DNA序列测定和同源性分析,鉴定菌株JH01为稻黄单胞菌水稻致病变种(Xanthomonas oryzae pv.oryzicola,Xoc)。Bacterial leaf streak （ BLS） is one of the most important diseases of rice, and as the climate warming; it has a worsening tendency to occur year by year that greatly affect the production.In 2013, BLS broke out in some rice planting areas of Jinhua City, Zhejiang Province, and caused decline over 30%of rice yield in some field with serious disease.Adopting the method of plant tissue pathogen isolation from pathogenic leaves, 6 bacterial strains were isola-ted and purified, the typical strain JH01 was selected to inoculate the rice seedlings to validate Koch ’ s law.The dis-ease symptoms of inoculation were consistent with the disease occurred in nature , and the same strain was rei-solated from the infected rice leaves.And proved that strain JH01 was the pathogen of rice BLS.The strain JH01 was charac-terized as Xanthomonas o ryzae pv.oryzicola by morphological observation, routine physiological and biochemical indi-ces tests, and 16S rDNA sequences and homology analyses.

关 键 词：水稻 细条病 分离 鉴定 稻黄单胞菌 

分 类 号：Q939.96[生物学—微生物学]