门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯的发酵条件优化  

Optimization of Fermentation Conditions for Medium-Chain-Length Polyhydroxylalkanoate Synthesis by Pseudomonas mendocina NK-01

作　　者：段静静[1,2] 郭文斌[2] 张弛[2] 王媛媛[2] 宫婷[2] 宋存江[2] 

机构地区：[1]厦门大学环境与生态学院,福建厦门361102 [2]南开大学生命科学学院,天津300071

出　　处：《微生物学杂志》2015年第4期40-45,共6页Journal of Microbiology

基　　金：国家自然科学基金项目(31070039)

摘　　要：为了提高PHAMCL在门多萨假单胞菌NK-01中的积累,采用单因素实验和正交实验确立了发酵生产PHAMCL的最佳条件,即以PHA产量为指标的最佳发酵条件为15 g/L葡萄糖浓度、C/N=50、发酵时间48h,该条件下获得产量0.8 g/L以上的PHA;以PHA占菌体干重百分含量为指标的最佳发酵条件为10 g/L葡萄糖浓度、C/N=60、发酵时间48 h,该条件下获得占菌体干重50%以上的PHA。该研究将为门多萨假单胞菌NK-01用于PHAMCL的规模化生产提供理论依据。In order to improve the accumulation of PHAMcL in vivo, single factor and orthogonal experiments were adopted to study the optimization fermentation conditions for Pseudomonas mendocina NK-01. When PHAMCL output was taken as an index, under optimized fermentation conditions at 15 g/L glucose, with C/N ratio at 50 and fermented for 48 h, produced over 0.8 g/L of PHAMcL. When the index was turned to PHAMcL percentage content of dried cell weight （DCW）, the best fermentation conditions were 10 g/L glucose, with C/N ratio at 60 and fermented for 48 h, the PHAMcL output was over 50% of DCW. This study will provide theoretic foundations for large scale production of PHAMcL by P. mendocina NK-01.

关 键 词：门多萨假单胞菌 聚羟基脂肪酸酯 正交实验 

分 类 号：Q815[生物学—生物工程]