复方黄芪对鸡新城疫灭活疫苗免疫效果的影响  

Effects of Compound Astragalus on Immune Efficacy of Inactivated Vaccine against Newcastle Disease in Chickens

作　　者：甘辉群[1] 刘明生[1] 贺生中[1] 傅宏庆[1] 谭菊[1] 徐勇 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]江苏苏鹏禽业发展有限公司,江苏海安226661

出　　处：《动物医学进展》2015年第10期69-72,共4页Progress In Veterinary Medicine

基　　金：江苏农牧科技职业学院自然科学项目(NSFPT1416);江苏苏鹏禽业发展有限公司横向合作课题

摘　　要：为探讨复方黄芪制剂对鸡新城疫灭活疫苗免疫效果的影响,将300只1日龄海兰褐雏鸡随机分成Ⅰ组(低剂量添加组)、Ⅱ组(中剂量添加组)、Ⅲ组(高剂量添加组)、Ⅳ组(阴性对照组)和Ⅴ组(阳性对照组)。7日龄时,除阴性对照组不免疫外,其余各组鸡均颈背部皮下注射新城疫灭活疫苗,0.25mL/羽,同时Ⅰ、Ⅱ和Ⅲ组分别按10、20、40g/kg体重,灌服复方黄芪制剂,每天1次,连续5d。然后在免疫后第7、14、21、28、35天时,从各组中随机选取8只健康鸡无菌采集心血1.0mL,分离血清,用于鸡新城疫HI抗体、SOD和IFN-γ的测定。结果表明,新城疫HI抗体效价,血清中SOD和IFN-γ的活性或浓度,复方黄芪中剂量组均高于其余各组,且与低剂量添加组、阳性对照组相比,呈显著(P<0.05)或极显著差异(P<0.01)。说明复方黄芪按20g/kg体重的剂量给雏鸡口服,能显著提高雏鸡的新城疫HI抗体水平,同时也能提高雏鸡SOD和IFN-γ的水平。To investigate the effects of compound Astragalus preparation on the immune efficacy of the in-activated Newcastle disease vaccine in chickens,300 one day-old Hyline brown chickens were randomly di-vided into groupⅠ（low-dose group）,group Ⅱ （moderate-dose group ）,group Ⅲ （high-dose group ）,groupⅣ （negative control group）and group Ⅴ（positive control group ）.When the chickens were 7 days of age, they were subcutaneously injected with the inactivated Newcastle disease vaccine except the negative con-trol group,each of 0.25 mL.Meanwhile,the chickens in the group Ⅰ,group Ⅱ and group Ⅲ were orally gavaged with the compound Astragalus preparations as 10 g/kg,20 g/kg and 40 g/kg body weight,respec-tively,once a day for 5 consecutive days.Then at the 7 d,14 d,21 d,28 d and 35 d after immunization,eight healthy chickens were randomly selected from each group and 1.0 mL blood were collected,and sera were separated for the detection of ND-HI antibodies,SOD and IFN-γ.The results were as follows：Newcastle disease HI antibody levels,serum activity or concentration of SOD and IFN-γin the compound Astragalus moderate-dose group were higher than that of other groups,and compared with the low-dose group and the positive control group,there were significant or very significant differences.The study showed that the compound Astragalus preparations,orally gavaged with 20 g/kg body weight dose,can significantly im-prove ND-HI antibody levels and the seral activities or concentrations of SOD and IFN-γin chickens.

关 键 词：复方黄芪 鸡新城疫灭活疫苗 鸡新城疫HI抗体 超氧化物歧化酶 Γ干扰素 

分 类 号：S853.74[农业科学—临床兽医学]