癌症标记蛋白AGR2的原核表达及纯化  

Prokaryotic Expression and Purification of Cancer Maker Protein AGR2

作　　者：刘文杰[1] 魏龙刚[1] 陶进[1] 吕忠显[1] 

机构地区：[1]厦门大学药学院,福建厦门361102

出　　处：《动物医学进展》2015年第10期81-84,共4页Progress In Veterinary Medicine

基　　金：福建省自然科学基金项目(2012J01155);中央高校基本科研业务费项目;厦门大学药学院院长基金项目

摘　　要：前梯度蛋白2(anterior gradient 2,AGR2)是一种分泌蛋白,在多种腺癌中过表达,是一个潜在的肿瘤诊断和预后判断的标志物。用质粒pGEX4T-1构建AGR2蛋白原核表达载体(pGEX4T-1-△SPAGR2),IPTG诱导表达,考马斯亮蓝染色和Western blot验证纯化的AGR2蛋白。结果表明,利用大肠埃希菌培养,原核表达载体(pGEX4T-1-△SP-AGR2)在25℃、0.1mmol/L IPTG诱导16h后AGR2蛋白大量表达,分离纯化得到具有生物活性的成熟AGR2蛋白,这对于开展AGR2生物机制的研究,以及开发AGR2的诊断和治疗试剂具有重要的意义。Anterior gradient-2 （AGR2）is a secreted protein and overexpressed in many tumor tissues of glands.Thus,AGR2 is considered to be a promising biomarker for early diagnosis and prognosis on cancer. It was used to express AGR2 by prokaryotic expresion vector pGEX4T-1-△SP-AGR2 in E .coli.After in-duction with IPIG,the protein was assessed with Coomassie bright blue gel and Western blot.The results showed that the AGR2 vector was induced with IPTG at 25℃ for 16 hours to get high expression of pure and bioactive AGR2 protein.Our results suggest potential uses in the study the mechanism of AGR2 as a secreted prote of the tumor.In a broader sense,they also has important theoretical and practical significance for early diagnosis and prognosis of cancer.

关 键 词：前梯度蛋白2 原核表达 分离纯化 AGR2 

分 类 号：S852.33[农业科学—基础兽医学]