大孔树脂纯化展毛地椒总黄酮工艺研究  

Purification Technology of Total Flavonoids from Thymus by Macroporous Resin

作　　者：豆浩然 倪健[1] 葛亮[2,3] 曹飒丽[1] 林龙飞[1] 夏振文[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]新疆名医名方与特色方剂学重点试验室,新疆乌鲁木齐830011 [3]新疆医科大学中医学院,新疆乌鲁木齐830011

出　　处：《辽宁中医杂志》2015年第9期1726-1729,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：北京市"十病十药"研发项目(Z131100002513011);北京中医药大学复方中药制药创新团队基金项目(2011-CXTD-13)

摘　　要：目的:优选大孔树脂纯化展毛地椒总黄酮的工艺参数。方法:采用紫外分光光度法测定总黄酮含量,以总黄酮的吸附率和解吸率为指标,考察大孔树脂型号、上样液浓度、上样流速、洗脱剂种类及其用量等影响因素,优选最佳分离纯化条件。结果:AB-8树脂具有较好的吸附率和解吸率,最佳纯化工艺:上样液浓度为0.2 g/m L,上样流速为2 BV/h,上样体积为2 BV,洗脱溶剂为50%乙醇,洗脱体积为5 BV,径高比为1/6,富集物中展毛地椒总黄酮含量达到26.32%。结论:AB-8型大孔树脂能较好地用于分离纯化展毛地椒总黄酮。Objective：To optimize the purification technology parameters of total flavonoids from Thymus using macroporous resins. Methods：The content of total flavonoids in Thymus was determined by ultraviolet spectrophotometry spectrophotometer. With the adsorption and desorption capacity as index, purification technology was optimized by investigating the type of macro- porous resin, concentration of sample liquid ,flow speed for adsorption, type and amount of eluent solvent and other indicators. Re- suhs：AB -8 macroporous resin showed the good property for the absorption and desorption capacity for total flavonoids in Thy- mus. The optimized conditions were as follows ： the concentration of sample liquid was 0.2 g/mL; the flow rate was 2 BV/h ; the e- lution solvent was 50% ethanol;the volume of elution solvent was 5 BV and the ratio of diameter to height was 1/6. Under the op- timized purification condition, the content of total flavonoids was up to 26. 32% in the enriched product. Conclusion：AB -8 macroporous resin can be effectively applied to purify total flavonoids from Thymus.

关 键 词：展毛地椒 总黄酮 大孔树脂 纯化工艺 

分 类 号：R284[医药卫生—中药学]