小儿咽扁颗粒中野鸢尾黄素和白射干素含量测定方法研究  

Study on Determination Method of Irigenin and Dichotomitin in Xiaoer Yanbian Granule

作　　者：李锁[1] 张晓瑞[1] 尤献民[2] 邸子真[2] 李国信[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《辽宁中医杂志》2015年第9期1733-1735,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"十二五"重大新药创制项目资助项目(2012ZX09303-017);国家自然基金资助项目(81273927);国家临床重点专科建设项目;国家中医药管理局临床中药学重点学科;辽宁中医药大学杏林学者青蓝工程李国信工作室项目

摘　　要：目的：建立小儿咽扁颗粒中野鸢尾黄素、白射干素含量的测定方法。方法：L9（34）正交设计优选供试液制备方法，采用XB—C19色谱柱（4．6mm×250mm，5μm），流动相为乙腈（B）－0．1％磷酸水溶液（A）梯度洗脱，检测波长为265nm，流速为1．0mL·min^-1，柱温为30℃。结果：野鸢尾黄素、白射干素分别在2．560×10^-2～0．6400μg（r=0．9995）和5．240×10^-3～0．130μg（r=1．0000）范围内线性关系良好。精密度、重现性、稳定性的RSD均小于2％，野鸢尾黄黄素及白射干素的平均加样回收率分别为95．93％和96．19％。结论：所采用方法可靠、准确、重现性好，可用于该制剂的含量测定及质量控制。Objective ： A method for determination of irigenin and dichotomitin in Xiaoer Yanbian Granule was set up. Methods ： L9 （ 34 ） orthogonal design was used to optimize the selected liquid preparation methods. The separation was performed on XB - C18 （4.6 mm × 250 mm ,5 μm）column with the mobile phase consisting of phosphoric acid（A） -acetonitrile （B） at the flow rate of 1.0 mL · min ^-1. The column temperature was 30 ℃ and the detection wavelength was 265 nm. Results ： The method had effective separation of the irigenin and dichotomitin. The linear ranges of irigenin and diehotomitin were 2. 560 × 10^ -2 ～ 0.6400 μg（ r = 0. 9995 ） and 5. 240 × 10^-3 -0. 1310 μg（ r = 1. 0000）, respectively. The RSD of precision, reproducibility and stability was 〈 2%. The average recoveries of two constituents were 95.93% and 96.19%. Conclusion：The method is simple and reliable with good reproducibility and can be to used tot determine and control the quality of Xiaoer Yanbian Granule.

关 键 词：小儿咽扁颗粒 野鸢尾黄素 白射干素 含量测定 

分 类 号：R284[医药卫生—中药学]