脾虚痰浊证大鼠主动脉cAMP/PKA信号通路变化的实验研究  

Experimental Study of c AMP / PKA Signaling Pathway Changes in Aorta of Rats with Spleen Phlegm

作　　者：冷雪[1] 杨关林[1] 贾连群[1] 宋囡[1] 朱美林[1] 王洁明[1] 臧安缘 

机构地区：[1]辽宁中医药大学中医脏象理论及应用教育部重点实验室,辽宁沈阳110032

出　　处：《辽宁中医杂志》2015年第9期1789-1791,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划("973"计划)资助项目(201133CB531702);国家自然科学基金青年基金项目(81202834)

摘　　要：目的:观察脾虚痰浊证大鼠主动脉c AMP/PKA信号通路变化,探讨其信号通路变化与脾虚痰浊证的关系。方法:16只SD大鼠,每组8只,随机分为空白对照组、脾虚痰浊组。全自动生化分析仪检测血清TC、TG、LDL-C、HDLC水平,ELISA法检测血浆中c AMP浓度,RT-PCR和Western blot检测肝脏GP、CREB、PKA和PHK蛋白表达水平。结果:与空白对照组相比,TG含量显著增高,主动脉c AMP活性显著降低,GP、CREB、PKA、PHK mRNA含量显著下降,GP、CREB、PKA、PHK蛋白表达显著下降。结论:脾虚痰浊证大鼠主动脉c AMP/PKA信号通路的改变可能与调控血脂代谢相关基因GP、CREB、PKA、PHK的表达有关。Objective ： To observe the aorta cAMP/PKA signal pathway changes in the rats with spleen phlegm and discuss the relationship between the signal pathway changes and spleen phlegm. Methods：Sixteen SD rats were randomly divided into con- trolled group and spleen phlegm group with eight in each. Detect serum TC, TG, LDL - C and HDL - C levels with an automatic biochemical analyzer. Detect cAMP concentrations in the plasma with the ELISA method. Apply the RT - PCR and Western blot to detect CREB, GP, PKA and PHK protein content levels in the liver. Results ： Compared with the controlled group, the TG levels were significantly increased and aortic cAMP levels were significantly decreased. GP,CREB,PKA and pI-IK mRNA were signifi- cantly decreased and GP, CREB, PKA and PHK protein contents were significantly decreased. Conclusion：Aorta cAMP/PKA sig- nal pathway changes in the rats with spleen phlegm may be associated with the regulation of lipid metabolism genes GP, CREB, PKA and PHK.

关 键 词：脾虚痰浊证 CAMP/PKA 主动脉 信号通路 

分 类 号：R241.6[医药卫生—中医诊断学]