大气压低温等离子体与胸腺嘧啶脱氧核苷的作用  

Atmospheric-pressure Plasma Interaction with Thymidine in Aqueous Solution

作　　者：杨思泽[1] 杨周斌 周仁武[1] 张先徽[1] 

机构地区：[1]厦门大学物理与机电工程学院,电子科学系,电磁声学研究院,福建省等离子体与磁共振研究重点实验室,厦门361000

出　　处：《高电压技术》2015年第9期2837-2843,共7页High Voltage Engineering

基　　金：福建省自然基金(2014J01025);国家自然科学基金(11275261)~~

摘　　要：胸腺嘧啶(Thymine)是遗传物质的组成成分之一,为了研究等离子体对遗传胸腺嘧啶(Thymine)的作用过程,利用大气压等离子体对作为遗传物质的胸腺嘧啶(Thymine)及对应的胸腺嘧啶脱氧核苷(Thymidine)进行处理,为了避免空气对遗传物质的影响,采用水中放电装置进行了相关研究。研究结果表明:不同气体等离子体处理后,胸腺嘧啶脱氧核苷会分解为胸腺嘧啶,且氮气等离子体处理时分解速率快于氩气等离子体处理;另外,空气等离子体与氧气等离子体处理后,胸腺嘧啶脱氧核苷会分解产生两种新产物,分别为胸腺嘧啶+OH和胸腺嘧啶脱氧核苷+OH。该研究对了解大气压等离子体对细菌失活的机制具有一定指导作用,同时对等离子体与生物体作用有着积极的指导意义。The cold atmospheric-pressure plasmas have great application prospects in the fields of biomedicine, such as killing virus, sterilization, cancer cells ablation, and surface treatment of organism tissue, etc. Some reports have indicated that the cold atmospheric-pressure plasmas will modify the genetic material （RNA and DNA） in organism tissue. Thymine is one of the components of genetic material. In this paper, in order to study the effect of plasma on genetic Thymine process, Thymine and Thymidine in the aqueous solution were treated by plasma. In order to avoid the effect of air on experiments, a new discharge device was designed to generate large-area plasma in aqueous media. The results show that,after different gas plasma processing, Thymidine decomposes for thymine; After air plasma and oxygen plasma treatment, Thymidine can produce two new decomposition products： Thymine ＋OH and Thymidine ＋OH. The study of DNA degration caused by plasma has certain instruction function in biomedicine.

关 键 词：大气压冷等离子体 含氧自由基 胸腺嘧啶(Thymine) 胸腺嘧啶脱氧核苷(Thymidine) pH值 色谱 质谱 

分 类 号：Q68[生物学—生物物理学]