草酸钙结石小鼠模型的构建  

Establishment of oxalate crystals formation in mouse kidney

作　　者：吴柏霖[1] 郭小林[1] 王博涵[1] 刘军[1] 夏丁[1] 杨欢[1] 陈志强[1] 叶章群[1] 徐华[1] 余虓[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《中华实验外科杂志》2015年第9期2292-2294,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81000292）

摘　　要：目的 构建小鼠肾脏草酸钙结石模型.方法 使用8周龄雄性C57BL/6小鼠,每天接受不同草酸代谢前体物质腹腔注射处理,包括乙醇酸80 mg/kg和120 mg/kg、乙醛酸80 mg/kg和120 mg/kg、草酸钠140 mg/kg和210 mg/kg,观察小鼠生存情况及体质量变化,肾脏切片行Pizzolato＇s染色观察肾脏内草酸钙晶体形成情况.结果 乙醇酸80 mg/kg和乙醛酸80 mg/kg腹腔注射对小鼠的生存影响最小,乙醛酸80 mg/kg腹腔注射对小鼠的体质量影响最小.乙醇酸腹腔注射无法在小鼠肾脏形成草酸钙晶体,而乙醛酸和草酸钠可在肾脏切片上观察到晶体形成.草酸钙晶体在0～6d逐渐增加,6～9d处于平台期,9～15d逐渐减少.结论 乙醛酸80 mg/kg腹腔注射是诱导小鼠肾脏草酸钙结石的最佳剂量,小鼠存在结石的形成期和清除期.Objective To establish a mouse model of calcium oxalate urolithiasis.Methods experimental animals were C57BL/6 mice.All the mice accepted intraabdominal injection treatment,using glycolate 80 mg/kg or 120 mg/kg per day,glyoxylate 80 mg/kg or 120 mg/kg per day,sodium oxalate 140 mg/kg or 210 mg/kg per day.The survival rate and weight were measured.The stone formation were evaluated by Pizzolato＇ s staining.Results Glycolate 80 mg/kg per day and glyoxylate 80 mg/kg per day intraabdominal injection exerted minor influence on mice survive.Glyoxylate 80 mg/kg per day intraabdominal was better than glycolate.Glycolate intraabdominal injection couldn＇ t induce stone formation in kidney,while glyoxylate and sodium oxalate could form crystals in the mice kidneys.The oxalate crystals increased in the first 6 days and remained on day 9.Then the crystals decreased on day 9-15.Conclusion Glyoxylate 80 mg/kg per day intraabdominal injection is the best stone-formation reagent.The calcium oxalate crystals in mouse has a stone-forming phase and a stone-eliminating phase.

关 键 词：草酸钙结石 小鼠模型 乙醛酸 

分 类 号：R691.4[医药卫生—泌尿科学]