萼脊兰总RNA提取方法的比较研究  

Comparison research of extraction method of total RNA from sedirea japonica

作　　者：蒋素华[1] 李艳辉 王洁琼[3] 梁芳[1] 王默霏[1] 崔波[1] 

机构地区：[1]郑州师范学院生物工程研究所,河南郑州450044 [2]封丘县农业局,河南新乡453300 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2015年第4期468-471,共4页Journal of Henan Agricultural University

基　　金：郑州市科技计划项目(112PPTGY250-3)

摘　　要：采用Trizol法、CTAB法、RNA prep Pure Plant Kit方法分别从萼脊兰的根、叶、花葶、花萼、花瓣、唇瓣、柱头中提取总RNA,比较3种总RNA提取方法的优劣,分析所提取萼脊兰不同组织总RNA质量高低。结果显示:CTAB法提取的RNA得率最高,完整性好,凝胶电泳泳道无杂质,条带单一,可以满足荧光定量PCR的试验需要。The total RNA of sedirea japonica from the root, leaf, pedicel, sepal, petal, column and labellum was extracted by Trizol method, CTAB method and RNA prep Pure Plant Kit, respectively. The and quality of total RNA extracted from different tissues was compared and analyzed. The results showed that the best was CTAB method which had high yield, high integrity, clear and single strip in the gel eleetrophoresis, and could meet the needs of the real time quantitative PCR test.

关 键 词：萼脊兰 不同组织 总RNA提取方法 

分 类 号：Q786[生物学—分子生物学]