草乌及其炮制品的急性毒性实验研究  

Experimental Study on Acute Toxicity of the Aconiti kusnezo ffii Radix and Its Processed Products

作　　者：刘帅[1] 刘晓艳[1] 李妍[1] 杨畅[1] 刘秋雨[1] 李飞[1] 杜红[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国药物警戒》2015年第9期513-516,521,共5页Chinese Journal of Pharmacovigilance

基　　金：中医药行业科研专项项目(201107008);国家重点基础研究发展计划(973计划)(2009CB522805)

摘　　要：目的比较生草乌、药典法制草乌、诃子制草乌的粉末和水煎液及诃子草乌配伍煎煮液7种样品急性毒性大小,探讨草乌炮制减毒原理。方法采用灌胃给药的方法,分别测定7种草乌样品对小鼠半数致死量(LD50)或最大给药量。结果生草乌粉末、诃子制草乌粉末、诃子草乌配伍煎液、诃子制草乌煎液、生草乌煎液的LD50分别为0.7009、1.1690、2.5600、4.0000、15.3200 g·kg-1;药典法制草乌粉末、药典法制草乌水煎液最大给药量分为20 g·kg-1和64 g·kg-1。结论生草乌毒性较大,生品经炮制或水煎煮后毒性均有不同程度降低。药典法制草乌无论粉末还是水煎液均无明显毒性。Objective To discuss the principle of the processing method of raw Aconiti kusnezoffii Racfix by comparing the acute toxicity of seven kinds of samples made with Aconiti kusnezoffii Radix or its processed products. Methods The median lethal dose （LD50） or the maximum dose of seven kinds of samples were measured by gavage method on mice. Results The LD50 of powder of raw Aconiti kusnezoffii Radix, powder of Aconiti kusnezoffii Radix processed with Chebulae Fructus, decoction of compatibility of Aconiti kusnezoffii Rachx and Chebulae Fructus, decoction of Aconiti kusnezoffii Radix processed with Chebulae Fructus, decoction of raw Aconiti kusnezoffii Radix decoction was respectively 0.7009, 1.1690, 2.5600, 4.0000, 15.3 200 g·kg-1. The MTD of powder and decoction of Aconiti kusnezoffii Radix processed by pharmacopoeia method was respectively 20 g·kg-1 and 64 g·kg-1. Conclusion Powder of raw Aconiti kusnezoffii Rad/x had obvious toxicity, the acute toxicity of raw Aconiti kusnezoffii Radix was reduced to varying degrees. Powder and decoction of Aconiti kusnezoffii Radix processed by pharmacopoeia method had no obvious toxicity.

关 键 词：草乌 炮制 诃子制草乌 急性毒性 半数致死量 

分 类 号：R965[医药卫生—药理学]