藏药佐太对大鼠CYP1A2和NAT2活性的影响  

Effects of Tibetan Medicine Zuotai on the Activities of CYP1A2 and NAT2 in Rats

作　　者：范雪汝[1] 朱俊博[1] 姚星辰[1] 袁明[1] 李向阳[1] 

机构地区：[1]青海大学医学院药学系,西宁810001

出　　处：《中国药房》2015年第28期3932-3934,共3页China Pharmacy

基　　金：青海大学医学院中青年科技项目(No.2011-KYY-06)

摘　　要：目的:研究藏药佐太对大鼠细胞色素氧化酶(CYP1A2)、药物代谢酶N-乙酰基转移酶2(NAT2)活性的影响。方法:将70只SD大鼠随机均分为正常对照(生理盐水)组和佐太低、中、高剂量(1.2、3.8、12 mg/kg)单次给药组和多次给药组(每天1次,连续12 d),分别ig给药。正常对照组、佐太单次给药组于第2天,佐太多次给药组于第13天分别ig给予咖啡因(25 mg/kg),5 h后采集尿液,按10 mg/ml加入维生素C。采用高效液相色谱法测定大鼠尿液中咖啡因代谢物5-乙酰氨基-6-甲酰氨基-3-甲基尿酸(AFMU)、1-甲基黄嘌呤(1X)、1-甲基尿酸(1U)、1,7-二甲基尿酸(17U)的含量,以(AFMU+1X+1U)/17U、AFMU/(AFMU+1X+1U)比值来反映CYP1A2和NAT2活性。结果:与正常对照组比较,佐太中剂量单次给药组及多次给药组、高剂量多次给药组大鼠(AFMU+1X+1U)/17U、AFMU/(AFMU+1X+1U)比值降低,即CYP1A2和NAT2活性降低,差异有统计学意义(P<0.05)。结论:佐太对大鼠CYP1A2和NAT2活性有明显抑制作用。OBJECTIVE：To study the effects of Tibetan medicine Zuotai on the activities of cytochrome oxidase （CYP1A2） and drug metabolism enzyme N-acetyltransferase 2（NAT2）in rats. METHODS：70 SD rats were equally randomized into a normal control （normal saline） group,the groups of single administration of low,middle and high-dose （1.2,3.8 and 12 mg/kg） Zuotai and the groups of multiple administrations thereof（once daily for 12 consecutive days）. The rats were given drugs ig. caffeine（25 mg/kg）was given ig to the rats in the normal control group and the groups of single administration on the 2nd day,and to those in the groups of multiple administrations on the 13th day. 5 h later,their urine was collected and added with vitamin C based on 10 mg/ml. High performance liquid chromatography （HPLC） was adopted to determine the cafeine metabolites contents of 5-acetami-do-6-formamido-3-methyl-uric acid（AFMU）,1-methylxanthine（1X）,1-methyl-uric acid（1U）and 1,7-dimethyl uric acid（17U） in rats’urine,and the activities of CYP1A2 and NAT2 were reflected through （AFMU＋1X＋1U）/17U and AFMU/（AFMU＋1X＋1U）. RESULTS：Compared with normal control group,the（AFMU＋1X＋1U）/17U and AFMU/（AFMU＋1X＋1U）in rats were de-creased,namely the activities of CYP1A2 and NAT2 were lower in the groups of single administration of middle-dose Zuotai and multiple administrations of middle and high-dose Zuotai than in the normal control group. There was statistical difference （P〈0.05）. CONCLUSIONS：Zuotai can obviously inhibit the activities of CYP1A2 and NAT2 in rats.

关 键 词：藏药 佐太 N-乙酰基转移酶2 细胞色素氧化酶 大鼠 

分 类 号：R966[医药卫生—药理学]