舟山黄海葵粗提物抗人肺癌SPC-A1细胞的活性研究  

Study on the Antitumor Activity of Anthopleura xanthogrammica Crude Extract on SPC-A1 Cells

作　　者：张亚茹[1] 罗李王 杨最素[1] 赵玉勤[1] 余方苗[1] 王飞[1] 丁国芳[1] 

机构地区：[1]浙江海洋学院食品与医药学院/浙江省海洋生物医用制品工程技术研究中心,浙江舟山316022

出　　处：《中国药房》2015年第28期3947-3950,共4页China Pharmacy

摘　　要：目的:研究舟山黄海葵(黄海葵)粗提物体外抗人肺癌SPC-A1细胞的活性。方法:通过反复冻融、丙酮沉淀等方法制备黄海葵粗提物。以质量浓度分别为0(空白对照)、0.625、1.25、2.5 mg/ml的黄海葵粗提物作用于SPC-A1细胞24、48、72 h后,采用MTT法测定细胞活力,计算增殖抑制率、半数抑制浓度(IC50);24 h后分别通过HE染色、AO/EB荧光染色观察SPC-A1细胞形态学变化。结果:MTT法测定结果表明,黄海葵粗提物对人肺癌SPC-A1细胞具有显著的增殖抑制作用,随着黄海葵粗提物质量浓度的增加以及作用时间的延长,其对SPC-A1细胞的抑制率逐渐增高;作用24、48、72 h后的IC50分别为1.81、1.32、1.18 mg/ml;HE、AO/EB染色结果显示,相较于空白对照组,加药组细胞出现了细胞形态缩小、胞质内出现空泡、核固缩和核消失等明显的凋亡形态学改变。结论:舟山黄海葵粗提物对人肺癌SPC-A1细胞的增殖具有抑制作用。OBJECTIVE：To study the antitumor activity of Anthopleura xanthogrammica crude extract on human lung cancer SPC-A1 cells in vitro. METHODS：A. xanthogrammica crude extract obtained by the methods of repeated freezing and thawing,ac-etone precipitation. After treated with crude extract 0（blank control）,0.625,1.25 and 2.5 mg/ml for 24,48 and 72 h,the activity of SPC-A1 cells were measured by MTT assay. The growth inhibition rate and IC50 were also calculated. 24 h later,the morphologi-cal changes of SPC-A1 cells were observed by HE staining and AO/EB fluorescence staining. RESULTS：MTT assay showed that A. xanthogrammica crude extract has significant inhibitory effect on the proliferation of human lung cancer SPC-A1 cells;with the increasing of the concentration and the extension of the time,the inhibitory rate was increased. Its 24 h,48 h ,72 h IC50 were 1.81,1.32 and 1.18 mg/ml. HE staining and AO/EB staining appeared obvious morphological changes of apoptosis that cell mor-phology narrowed,vacuoles arose in the cytoplasm,karyopyknosis and part of nuclear disappearance occurred. CONCLUSIONS：A. xanthogrammica crude extract has an inhibitory effect on the proliferation of human lung cancer SPC-A1 cells.

关 键 词：黄海葵粗提物 SPC-A1细胞 增殖 凋亡 

分 类 号：R979.1[医药卫生—药品] Q279[医药卫生—药学]