冰片-葛根素脂质体的制备及其脑靶向性研究  

Preparation of Borneol-puerarin Liposomes and Study on Its Brain-targeting

作　　者：刘薇芝[1] 胡汉昆[1] 刘萍[1] 刘巍[1] 郑艳[1] 

机构地区：[1]武汉大学中南医院药学部,武汉430071

出　　处：《中国药房》2015年第28期3964-3966,共3页China Pharmacy

基　　金：湖北省自然科学基金资助项目(No.2012FFB04329)

摘　　要：目的:制备冰片-葛根素脂质体,并考察其脑靶向性。方法:采用薄膜分散超声法制备冰片-葛根素脂质体,透射电子显微镜观察脂质体形态,激光粒度仪测定其粒径和Zeta电位,葡聚糖凝胶过滤法测定包封率。以葛根素注射液为参照,以相对摄取率和峰浓度比考察小鼠尾iv葛根素脂质体、冰片-葛根素脂质体的脑靶向性。结果:制备的冰片-葛根素脂质体呈圆形或类圆形,平均粒径为226 nm,多分散指数为0.263,Zeta电位为-21.3 m V,包封率为(65.32±2.13)%。以葛根素注射液为参照,葛根素脂质体和冰片-葛根素脂质体的相对摄取率分别为1.68、2.58,峰浓度比分别为1.15、1.42。结论:成功制得具有较好脑靶向性的冰片-葛根素脂质体。OBJECTIVE：To prepare borneol-puerarin liposomes,and to investigate its brain-targeting. METHODS：Film dis-persion ultrasonic method was used to prepare borneol-puerarin liposomes. The morphology of liposomes was observed by TEM;the particle size and Zeta potential were measured by laser particle size analyzer;the entrapment efficiency were measured by sepha-dex gel filtration method. Compared with Puerarin injection,brain-targeting of borneol-puerarin liposomes and puerarin liposomes via intravenous injection of mice tail was studied with relative intake rate and peak concentration ratio. RESULTS：Borneol-puerarin liposomes were spherical or quasi-circular;its mean particle size,polydispersity index and Zeta potential were 226 nm,0.263 and-21.3 mV respectively. The entrapment efficiency were（65.32±2.13）%. Compared with Puerarin injection,relative intake rate of puerarin liposome and borneol-puerarin liposome were 1.68 and 2.58,and peak concentration ratio were 1.15 and 1.42. CONCLU-SIONS：Brain-targeted borneol-puerarin liposomes are prepared successfully.

关 键 词：冰片 葛根素 脂质体 脑分布 制备 

分 类 号：R943[医药卫生—药剂学]