星点设计-效应面法优化重楼皂苷类成分的提取工艺  

Optimization of the Extraction Technology of Saponins from Paris polyphylla with Central Composite Design-response Surface Method

作　　者：鞠建峰[1] 朱宗敏[1] 杜忠科[1] 

机构地区：[1]山东中医药大学附属医院药剂科,济南250014

出　　处：《中国药房》2015年第28期3967-3969,共3页China Pharmacy

基　　金：山东省中医药科技发展计划项目(No.2013-082)

摘　　要：目的:优化重楼皂苷类成分的提取工艺。方法:以重楼皂苷Ⅰ、重楼皂苷Ⅱ及重楼总皂苷的提取率为因变量,以乙醇体积分数、提取时间、溶剂倍量为自变量,进行多元线性回归及二项式拟合,用效应面法优选最佳工艺,并进行预测。结果:重楼皂苷类成分最优提取工艺为加入80%乙醇10倍量,回流提取2次,每次100 min。采用该提取工艺,重楼皂苷Ⅰ提取率分别为85.4%、82.7%、87.1%,重楼皂苷Ⅱ提取率分别为85.9%、81.3%、83.6%,重楼总皂苷提取率分别为89.5%、92.1%、90.3%(RSD均小于2.0%);总评价指标实测值为0.964 9,预测值为0.986 0,偏差率为2.14%。结论:星点设计-效应面法优选重楼皂苷类成分提取工艺,方法简单,结果可靠。OBJECTIVE：To optimize extraction technology of saponins from Paris polyphylla. METHODS：Using paris sapo-nin Ⅰ,paris saponin Ⅱand Paridis total saponin as dependent variables,using ethanol volume fraction,extraction time and sol-vent amount as independent variables,through multiple linear regression and binomial fitting,the extraction technology was opti-mized with response surface method and predicted. RESULTS：The optimized extraction technology of saponins from P. polyphylla was as follows as 10-fold of 80% ethanol,2 times reflux extraction,100 min each time. Under the extraction technology,the ex-traction rates of paris saponinⅠwere 85.4%,82.7% and 87.1%;those of paris saponinⅡwere 85.9%,81.3% and 83.6%;and those of Paridis total saponin were 89.5%,92.1% and 90.3%（all RSD〈2.0%）. Measured value was 0.964 9,predicted value was 0.986 0 and deviation rate was 2.14%. CONCLUSIONS：The central composite design-response surface method is simple and reliable for the optimization of extraction technology of saponins from P. polyphylla.

关 键 词：星点设计 效应面法 重楼 重楼皂苷Ⅰ 重楼皂苷Ⅱ 重楼总皂苷 提取工艺 

分 类 号：R284.2[医药卫生—中药学]