不同pH值条件下3种大环内酯类抗菌药物的抗菌活性及耐药性  

Effect of different pH on antibacterial activity and drug resistance of macrolide antibiotics

作　　者：高慧莉[1] 罗光明[2] 

机构地区：[1]第三军医大学第一附属医院药剂科,重庆400038 [2]第三军医大学第一附属医院呼吸科,重庆400038

出　　处：《检验医学与临床》2015年第18期2690-2693,共4页Laboratory Medicine and Clinic

摘　　要：目的不同pH值条件下3种大环内酯类药物对金黄色葡萄球菌和化脓性链球菌的耐药性和最小抑菌浓度(MIC)。方法在pH6.0、pH7.0和pH8.0条件下,采用肉汤稀释法分别检测大环内酯类抗菌药物对临床分离金黄色葡萄球菌和化脓性链球菌MIC及细菌耐药性。结果随着pH值的增加,大环内酯类抗菌药物对金黄色葡萄球菌和化脓性链球菌的MIC值均有不同程度的降低,并呈一定的依赖效应,其中罗红霉素和阿奇霉素的抑菌活性强于红霉素;体外诱导试验表明,在所有试验pH值条件下,MIC值均有所增加,但是随着pH值的增加细菌的MIC值逐渐降低。结论大环内酯类抗菌药物的MIC随培养基pH值升高而逐渐降低,在一定pH值范围内大环内酯类抗菌药物可提高金黄色葡萄球菌和化脓性链球菌的抗菌作用。Objective To investigate the effect of staphylococcus aureus and streptococcus pyogenes on the drug resistance and the minimal inhibition concentration （MIC） of macrolide antibiotics under the different pH value conditions .Methods The broth dilution method was used to detect the drug resistance and MIC of macrolide antibiotics to clinically isolated staphylococcus aureus and streptococcus pyogenes .Results The drug resistance and MIC of macrolide antibiotics to staphylococcus aureus and streptococcus pyogenes were decreased with enhance of pH value in certainly dosedependent manner .In addition ,the inhibition activity of roxithromycin and azithromycin were stronger than that of erythromycin .The in vitro study indicated that the MIC values were increased in all the experiment pH conditions ,but the MIC values gradually were decreased with augment of pH values .Conclusion The MIC values of macrolide antibiotics are gradually decreased with augment of pH values in culture medium ,and macrolide antibiotics can improve the antibacterial activity of staphylococcus aureus and streptococcus pyogenes in certain pH values condition .

关 键 词：大环内酯类 抗菌活性 细菌耐药性 PH值 

分 类 号：R978.15[医药卫生—药品]