四个不结球白菜自交不亲和S单元型分子鉴定  

Molecular Identification of S Haplotypes in Four Self-Incompatible Germplasm Resources of Brassica campestris ssp. chinensis

作　　者：李霞[1] 胡侦华[1] 周国林[1] 汪爱华[1] 

机构地区：[1]武汉市蔬菜科学研究所武汉蔬菜展示中心,436400

出　　处：《湖北农业科学》2015年第16期3948-3951,共4页Hubei Agricultural Sciences

基　　金：国家大宗蔬菜产业技术体系项目(CARS-25-30);武汉市规模设施蔬菜关键技术集成与示范项目(20130217020110584)

摘　　要：利用已报道的SRK及SLG基因序列保守区设计的特异性引物,通过PCR扩增和克隆测序,结合生物信息学分析方法对4份国外引进的不结球白菜(Brassica campestris ssp.chinensis Makino)自交不亲和材料的S单元型进行分子鉴定。结果表明,不亲和材料A1包含的S位点基因序列与甘蓝(B.oleracea L.var.capitata L.)SLG-31的相似度为98%(E值是0);不亲和材料A2、A3包含Class I类及ClassⅡ类2种S单元型,S位点处于杂合状态。不亲和材料A4包含的S位点基因序列与青花菜(B.oleracea L.var.italica Plenck)单倍型BOI1 SRK蛋白基因具有98%的序列相似度,序列覆盖度达99%。Using the primers designed according to the conserved sequence of S locus kinase（SRK） gene and S locus glycoprotein（SLG） gene, by PCR amplification, combined with bio-informatics analysis the S haplotypes of 4 Brassica campestris ssp. chinensis Makino self-incompatibility material from overseas were identified. The results indicated that the A1self-incompatible line showed 98% similarity with SLG-31 gene in B. oleracea L. var. capitata L.; and the A2, A3 self-incompatible line both contained two types of S haplotype from Class I and Class Ⅱ, which showed that the S locus were at heterozygous state. The A4 self-incompatible line showed 98% similarity with BOI1 SRK gene in B. oleracea L. var. italica Plenck under 99% query coverage.

关 键 词：不结球白菜(Brassica CAMPESTRIS ssp.chinensis Makino) 自交不亲和 S单元型 分子鉴定 

分 类 号：S634.3[农业科学—蔬菜学] Q321.7[农业科学—园艺学]