风轮菜总黄酮不同极性部位的抗氧化作用研究  

Antioxidant Effect of Different Isolated polar Fractions in Total Flavones of Clinopodium chinense

作　　者：邢娜[1] 张海晶[1] 舒尊鹏[1] 许旭东[2] 朱寅荻[2] 孙桂波[2] 王秋红[1] 孙晓波[2] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室黑龙江中药及天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《世界中医药》2015年第8期1169-1172,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81374010);国家自然科学基金项目(编号:81173511)

摘　　要：目的:探讨风轮菜总黄酮的不同极性部位对缺氧/复氧诱导的H9c2心肌细胞损伤的保护作用。方法:建立缺氧/复氧H9c2心肌细胞损伤模型,对风轮菜总黄酮10%甲醇部位(2#)、20%甲醇部位(3#)、30%甲醇部位(5#)进行药物浓度筛选,采用MTT法测定细胞存活率。收集培养细胞及其上清液测定乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)的活性和丙二醛(MDA)的含量。结果:与正常对照组比,模型组H9c2心肌细胞经过缺氧4h复氧24h造模后,细胞活力显著降低。与模型组比较,2#、3#、5#给药组显著增加H9c2心肌细胞活力。与正常对照组比较,模型组的SOD、GSH-Px及CAT的活性均显著降低,而LDH活性、MDA含量均升高,2#、3#、5#给药组可呈浓度依赖性显著增加SOD、GSH-Px及CAT活性,降低LDH活性及MDA含量。结论:2#、3#、5#对缺氧/复氧诱导的H9c2心肌细胞损伤具有保护作用,其与增强细胞清除氧自由基能力、降低脂质过氧化物的产生有关。Objective：To explore the protective effect of different polar fractions in total flavones of Clinopodium chinenseon on H9c2 myocardial cell damage induced by hypoxia/reoxygenation（H/R）.Methods：The hypoxia/reoxygenation injury cardiomyo-cyte model was established and screened out parts with 1 0%（2#）,20%（3#）,30%（5 #）of carbinol.Cell viability was deter-mined by MTT method.We collected culture cell and its supernatant for detecting LDH,SOD,GSH-Px,CAT activity and MDA content.Results：In H/R group,after hypoxia 4 h and reoxygenation 24 h,the myocardial cell viability decreased significantly compared to control group.Compared with H/R group,2 #、3 #、5 #different isolated fragments in total flavones of Clinopodium chinense could increase cell viability.Compared to control group,SOD,GSH-Px,CAT were dropped remarkably,and LDH and MDA increased remarkably in H/R group.Compared to H/R group,2#、3#、5#medication groups decreased LDH and MDA signif-icantly,and increased SOD,GSH-Px,CAT significantly in a dose-dependent manner.Conclusion：2#、3#、5#can reduce myocar-dial damage induced by hypoxia/reoxygenation,the mechanism is related with the enhancement ability of myocardial cells to clear oxygen free radicals,and decrease the generation of lipid peroxide.

关 键 词：风轮菜总黄酮 H9C2 心肌细胞 缺氧/复氧 抗氧化作用 

分 类 号：R961[医药卫生—药理学]