高效液相色谱法同时测定竹叶中13种黄酮类化合物（英文）  

Simultaneous Determination of 13 Flavonoids in Bamboo Leaves by HPLC

作　　者：魏琦[1,2] 王淑英 汤锋 张华新[2] 喻谨 岳永德 

机构地区：[1]国际竹藤中心竹藤科学与技术重点实验室,北京100102 [2]国家林业局盐碱地研究中心,北京100091

出　　处：《林业科学》2015年第8期81-87,共7页Scientia Silvae Sinicae

基　　金：National Science and Technology Infrastructure Program(2012BAD23B03)

摘　　要：【目的】建立一种同时测定竹叶中13种黄酮类化合物（异荭草苷、荭草苷、异牡荆苷、牡荆苷、芹菜素、木犀草素、苜蓿素、7－甲氧基－苜蓿素、苜蓿素－7－ O －葡萄糖苷、芹菜素－7－ O －葡萄糖苷、Demethyltorosaflavone、芹菜素－7－O－葡萄糖－6″－O－鼠李糖苷、6－反式－（2″－O －α－鼠李糖基）乙烯基－5，7，3′，4′－四羟基黄酮）含量的高效液相色谱法，并用此方法对牡竹属10种竹种（麻竹、龙竹、牡竹、黄竹、梁山慈竹、花吊丝竹、云南龙竹、福贡龙竹、勃氏甜龙竹、版纳甜龙竹）竹叶黄酮类化合物进行分析比较，以期为黄酮类化合物的定性定量检测、牡竹属竹叶的开发利用及提取竹叶黄酮时的竹种选择提供参考。【方法】采用 HPLC梯度洗脱分离黄酮类化合物，通过仪器精密度、LOD值、LOQ值、日内稳定性、日间稳定性及添加回收率对方法进行验证。竹叶样品用70%乙醇超声提取，经石油醚萃取后采用 HPLC 进行检测。【结果】各黄酮类化合物标准品分离效果良好，保留时间在13.23～54.67 min之间。13种黄酮类化合物标准品在0.01～500 mg·L －1的线性范围内呈良好的线性关系，相关系数 R2在0.9996～1.0000之间。仪器精密度 RSD 在1.06%～2.55%之间， LOD 值在0.01～0.10 mg·kg －1之间，LOQ值在0.03～0.34 mg·kg －1之间。方法准确性、日间与日内精密度良好，日内稳定性 RSD在0.15%～0.67%之间，日间稳定性在0.44%～5.61%之间，13种黄酮类化合较稳定。7种黄酮类化合物的添加回收率高于70%，其余6种黄酮类化合物的添加回收率在39.76%～68.75%之间。采用此方法对牡竹属10种竹种竹叶黄酮类化合物进行分析，结果显示各竹种竹叶黄酮类化合物含量存在差异，除木犀草素、6－反式－（2″－O－α－鼠李糖基）乙烯基－5，7，3′，4′－四羟基黄酮和 DemethyltorosaflavoObjective]Most flavonoids possess a variety of medical and biological activities such as anti-free radical, antioxidant,antiaging,anti-inflammatory,etc. They are widely used in pharmaceutical,health products and cosmetic fields. The objective of this paper was to establish a HPLC method for simultaneously qualitative-quantitative analysis of 13 flavonoids （ isoorientin, orientin, vitexin, isovitexin, apigenin, luteolin, tricin, 7-methoxy-tricin, tricin-7-O-glucopyranoside, apigenin-7-O-glucopyranoside, demethyltorosaflavone, apigenin-7-O-glucopyranoside-6″-O-rhamnoside and 6-trans-（2″-O-α-rhamnopyranosyl）ethenyl-5,7,3′,4′-tetrahydroxyflavone ） in bamboo leaves,and leaf flavonoids in 10 species of Dendrocalamus （ D. latiflorus,D. giganteus,D. strictus,D. membranceus,D. farinosus,D. minor var. amoenus,D. yunnanicus, D. fugongensis, D. brandisii and D. hamiltonii ） were comparatively analyzed by the established method with the aims to provide a guidance for qualitative and quantitative analysis of flavonoids, the development and utilization of Dendrocalamus bamboo leaves and the selection of bamboo species for flavonoids extraction.[Method]The flavonoids were separated by HPLC gradient elution method,and the validation was guaranteed by accuracy of equipment,LOD,LOQ,intraday/interday precision and recovery rate. Bamboo leaves were extracted with 70% （ v/v） ethanol-water by ultrasonic extraction,and then tested by HPLC after petroleum ether extraction. [Result]A good separation was found for each flavonoid,the retention times were ranged from 13. 23 to 54. 67 min. The standard curves showed a good linearity in the corresponding ranges between 0. 01 and 500 mg·L -1 （ R2≥0. 999 6）. The equipment accuracy RSD were 1. 06% -2. 55%,LOD and LOQ ranged from 0. 01 to 0. 10 mg·kg -1 ,and from 0. 03 to 0. 34 mg· kg -1 ,respectively. The RSD values of instrument accuracy, and intraday/interday precision indicated that it was reasonable to analyze the samples within 5 days. The RSD of intraday an

关 键 词：黄酮类化合物 竹叶 牡竹属 

分 类 号：S781.41[农业科学—木材科学与技术]